My bad, yes, as JohnyM and Rik pointed out slanted plates won't work. I was mixing it up with plates in infinity space.

Epi whitelight illumination with the PA20x through coverslips will get massive reflections from the first coverslip surface and decrease contrast. Placing polarizers at the illumination/imaging light path will improve this, but will probably mess up the object colors. Just musing, but using thick co...

There's an unimplemented Nikon patent application that describes a spherical aberration compensator for both finite and infinite systems. The embodiment in fig.3 is actually a representation of a 20x NA0.75 objective and a compensator...coincidence? https://patents.google.com/patent/US5978155A/en?q=...

Thanks for the info, I think I've seen this in another form. The objective they're using here is a 100x, and yes achieving large flat field at high mags is difficult. So I'm kind of thinking this is some marketing hype. The CFI60 100x has already gone under 2 iterations since this publication in 200...

And, I think the PA20VC and normal PA20 are already rated at OFN25mm at this generation. Are there any literature regarding peripheral resolution for the PA20VC? P.S. I didn't mention the easiest way to use a high NA 0.17mm coverslip corrected objective on a bare specimen. Just place a high precisio...

Well, actually I've already asked my Nikon rep that question. Their answer is "No." Though I think this more of a non-disclosure problem. I can maybe do a quick stack on my cells with a confocal to confirm this, but no, I don't have time for the time being. Then, naturally we have to go back and exa...

Has anyone used a single mode optic fiber for scrambling lasers? I've only read descriptions in text, and it seems simple enough. Get a fairly long fiber coupled with a laser, make a few simple loops in it... and vibrate the loop at some frequency, and voila, you get despeckled light out. It should ...

Sadly, I did the test 2years ago, and at the time the criteria for testing was only to rule out a faulty objective for a quick return to the seller. I should have kept the slide. As Beatsy has pointed out, there is little meaning in measuring the depth without knowing the RI of the glass used since ...

1501-9398 and 0500-0087 are both corrected for the same thickness. I've tested all three variations with a used genuine flow-cell for the GaIIx.
1501-9398 and 0500-0087 are most likley the same, but with different inventory numbers by illumina for different machines.

Thanks for the experiments! I am lazy enough not to capture a few images. As for what kind of compensator they actually use on the MiSeq and other new machines, I have no idea since they never come up on the bay as a complete optical bench, and no way any sane facility manager would allow a guy with...

Hi Lou, My bad, I forgot that the GAIIx was a single surface TIRF microscope, (it only can excite the binded fluophores on the lower surface of the flow-cell.) so the correction must be for 300um(glass)+100um(liquid). I'm guessing later models including MiSeq which have multi-surface readout, incorp...

Those non-labeled PA20's are from the GAIIx's and the labelled PA20xs are for the MiSeq platform from illumina. (The internal code for the MiSeq is "BOLT" and you'll see that on some PA20's still in their shipping packages.) As for the 300 micrometer coverslip correction for the non-labeled versions...

Hi,Ed. I also have one of those type T DIC units, but mine doesn't have/need a auxillary lens for functioning. And yet I too have noticed something that looks like an auxillary lens in a 1976 catalog, but didn't think it to be an essential part. How does the image look like? BTW, I've been looking a...

Pau, thank you for the follow up. Pardon me for the FOV jargon, I have to be more diligent on my phrasing. Absolutely, I completely agree with you. Yes, if it were a APS-C camera I'd choose direct projection without hesitating. Wider FOV, better matching pixel pitch with low mag high NA images. And ...

Congrats on your purchase! The E600 is a beautiful instrument. About direct imaging using it, I doubt the rear port of the Y-IDP unit allows an image circle of 43mm(FF) direct through it. I've seen people mount APC-C(27mm diagonal), which nearly matches the intended maximum image circle of the objec...