Search found 93 matches

by abednego1995
Wed Feb 20, 2019 10:09 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4987

Thanks Chris! Working with flat large specimens, yes I think those cylinders will be a huge pain... perhaps some silicone tubing might work. Must experiment. I'm currently thinking of photographing things smaller than the front lens diameter so just making a fairly long cylinder tacked on the object...
by abednego1995
Mon Feb 18, 2019 8:09 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4987

Gosh, I'm so bad at verbally describing things! Well, I'm thinking of this kind of setup. http://www.photomacrography.net/forum/userpix/5919_IMG_1824_1.jpg Those ELWD objectives, even with the 60x have ample WDs. I've enjoyed using the non-BD versions in the past. But I'm currently mulling the possi...
by abednego1995
Sun Feb 17, 2019 11:28 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4987

Since you already have a nice setup, what happens if you add a small cylindrical diffuser just outside of the image illuminated with the BD light path? (Perhaps a miniscule semi-transparent sphere like a miniture ping-pong ball would work better?) I sometimes see star shaped points of light in BD im...
by abednego1995
Thu Feb 14, 2019 1:23 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4987

Splendid comparisons!
What are the magnifications you worked with here? I tend to see low mags in brightfield with lower contrast (probably internal reflections of the objective masking the sample reflections), but that improves with higher mags (higher NA).

Cheers,
John
by abednego1995
Mon Feb 11, 2019 11:12 pm
Forum: Equipment Discussions
Topic: Zeiss Axiomat question
Replies: 18
Views: 14578

Wow, a private Axiomat!! Congrats on your acquirement. Sadly the manuals and brochures of the Axiomat I have don't shed light upon what that mount is... From the 3 flanges and the seemingly narrow optical path within I'm guessing an ENG (B4) mount used in video cameras. There are various adapters fo...
by abednego1995
Wed Feb 06, 2019 10:34 pm
Forum: Equipment Discussions
Topic: DIY Nikon filter cube & Lomo filters
Replies: 16
Views: 8649

Hi, that's a super nice print! One thing, if you're going to use high wattage illuminators, you'll have to be careful with interference filters at the LOMO slot filter position. Of course it depends on how good the IR filter is at the lamphouse, but they don't like to be hot. Plus, in the original s...
by abednego1995
Sat Feb 02, 2019 2:57 am
Forum: Equipment Discussions
Topic: OK what about Zeiss standard DIC?
Replies: 35
Views: 9314

So when we have a Nomarski prism, it's the front interference plane of the objective-side prism that must coincide with the objective rear focal plane - not the prism itself. Correct? Exactly. I've had the same problems in the past with DIC schematics showing Wollaston prisms in a Nomarski setup. A...
by abednego1995
Wed Jan 30, 2019 7:25 pm
Forum: Equipment Discussions
Topic: OK what about Zeiss standard DIC?
Replies: 35
Views: 9314

Yup, the beta angle and section angle are the same and they determine the distance of the interference plane to the prism. I've tried it with different prisms for reflected DIC as objective prisms and none of them worked. I've also tried my Oly U-DICT at different positions trying to improve evennes...
by abednego1995
Wed Jan 30, 2019 12:35 am
Forum: Equipment Discussions
Topic: OK what about Zeiss standard DIC?
Replies: 35
Views: 9314

Well, the main parameters for making a Nomarski prism would be the apex angle (alpha) and the section angle (beta). Shear amount is dictated by the apex angle, and where the interference plane comes, by the crystal(mostly quartz) axis section angle. http://www.photomacrography.net/forum/userpix/5919...
by abednego1995
Sat Jan 26, 2019 11:24 pm
Forum: Equipment Discussions
Topic: LED power to the epi-illuminator
Replies: 18
Views: 11082

To align white light epi-illuminators for Koehler illumination, one could place a mirror at the objective side, focus on it, remove a ocular and inspect the conoscopic image at the BFP of the objective. You could also use a phase telescope. If you can see the LED die, or bonding wires in the image s...
by abednego1995
Fri Jan 25, 2019 9:23 pm
Forum: Equipment Exchange
Topic: SOLD FS: Nikon NCF objectives SOLD
Replies: 1
Views: 2542

As of 28th, Jan. 2019, ALL SOLD
Thanks for watching!
by abednego1995
Wed Jan 23, 2019 7:17 pm
Forum: Equipment Exchange
Topic: SOLD FS: Nikon NCF objectives SOLD
Replies: 1
Views: 2542

SOLD FS: Nikon NCF objectives SOLD

I'm currently clearing out my drybox and have some Nikon objectives for sale. Mostly Nikon biological, but if you have any particular objective of interest (i.e. metallurgical) I may have it available. Please ask. I'll ship from Japan using EMS. 1: Nikon NCF Plan Apo 60x NA0.95 Used, and has some de...
by abednego1995
Sat Jan 12, 2019 7:18 pm
Forum: Equipment Exchange
Topic: WTB: "Advanced Light Microscopy" by M.Pluta
Replies: 7
Views: 3383

John, thanks for the Polish version link. The cover looks better than the Elsevier version! Pity Elsevier doesn't carry reprints of the English version as they do for other legacy textbooks.
by abednego1995
Fri Jan 11, 2019 5:41 am
Forum: Equipment Exchange
Topic: WTB: "Advanced Light Microscopy" by M.Pluta
Replies: 7
Views: 3383

Thanks guys, I've seen those insane prices on Abebooks and Amazon.
That Optica Applicata link is interesting, I'll get some good reading time browsing those...
by abednego1995
Sat Jan 05, 2019 5:17 am
Forum: Equipment Exchange
Topic: WTB: "Advanced Light Microscopy" by M.Pluta
Replies: 7
Views: 3383

WTB: "Advanced Light Microscopy" by M.Pluta

Does anyone have a spare or unneeded copy (Vol1~3) of Advanced Light Microscopy by Maksymilian Pluta?