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by Olympusman
Thu Jan 09, 2014 9:46 am
Forum: Photography Through the Microscope
Topic: Hyaline cartilage
Replies: 2
Views: 1524

Hyaline cartilage

In my post about human lung tissue showing cancer cells, jswatts speculated that the blue masses might be cartilage. Here is an image of hyaline cartilage. The cartilage is in the center. The tissue to the right appears to be a cross section of muscle tissue. http://www.photomacrography.net/forum/us...
by Olympusman
Wed Jan 08, 2014 3:18 pm
Forum: Photography Through the Microscope
Topic: Human lung tissue showing cancer cells
Replies: 11
Views: 4055

Lung tissue

This is lung tissue but the slide is not marked as to whether it is human or not. I have a cartilage slide which I will post tomorrow so we can compare. http://www.photomacrography.net/forum/userpix/2226_Healthy_lung_tissue_1.jpg Since we are challeneged for winter specimens in the Northern Hemisphe...
by Olympusman
Wed Jan 08, 2014 9:09 am
Forum: Photography Through the Microscope
Topic: Calcium Oxalate on Garlic's skin
Replies: 11
Views: 2657

Garlic and onion skin

Very nice. Is this the dry paper-like skin on the bulb?
by Olympusman
Wed Jan 08, 2014 8:59 am
Forum: Photography Through the Microscope
Topic: Human Bone Cross Section
Replies: 4
Views: 4557

Silver stain

I found a procedure for silver staining online.
First fix the section with formaldehyde or gluteraldehyde for a couple of days and then immerse the section in 2% silver nitrate for two days.
Pau, the color balance with the One-Touch White Balance is working well so far.
by Olympusman
Wed Jan 08, 2014 8:55 am
Forum: Photography Through the Microscope
Topic: Human lung tissue showing cancer cells
Replies: 11
Views: 4055

Human lung

I have other slides of human human lung tissue which I will post. The others show large areas clear of tissue. The blue cells look very much like the cancer I see in a Caposis Sarcoma slide I have. I've been accumulating histology slides for several years through online auctions and have a diverse s...
by Olympusman
Tue Jan 07, 2014 12:10 pm
Forum: Photography Through the Microscope
Topic: Human Bone Cross Section
Replies: 4
Views: 4557

Human Bone Cross Section

Human Bone thin cross section silver stained. Bone and nerve tissue are most commonly silver stained. I have some silver nitrate in my darkroom and want to find a formula for silver stain when I get my microtome up and running.

Image
by Olympusman
Tue Jan 07, 2014 8:08 am
Forum: Photography Through the Microscope
Topic: White-Tailed Deer Hairs
Replies: 0
Views: 1352

White-Tailed Deer Hairs

On New Years Eve my wife struck and killed a four-point buck with her van. Lots of damage to the front end of her van. There were some hairs stuck in the damaged fender and I mounted a few in balsam on a slide. We live in a mountainous wooded area south of Allentown, Pennsylvania. The Pennsylvania G...
by Olympusman
Tue Jan 07, 2014 7:59 am
Forum: Photography Through the Microscope
Topic: Human lung tissue showing cancer cells
Replies: 11
Views: 4055

Color balance

Pau, I had been using a Custom White Balance at 4000 K on my scope. But now i am trying a One-Touch White Balance on my Olympus E-420. For those of you not familiar with One-Touch White balance, you are able to shoot a white frame with the camera and it saves the white balance as a neutral gray in t...
by Olympusman
Mon Jan 06, 2014 9:31 am
Forum: Photography Through the Microscope
Topic: Mouse Kidney
Replies: 0
Views: 456

Mouse Kidney

Image
by Olympusman
Mon Jan 06, 2014 9:25 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: comparison
Replies: 75
Views: 15459

f8

NikonUser is recommending f8 over f32 because at f32 lens diffraction can seriously wipe out detail. The sweet spot on most lenses is usually a middle stop -- f8 or f11. You will have to have more shots in your stack, but the detail will be much finer.
by Olympusman
Mon Jan 06, 2014 9:18 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: comparison
Replies: 75
Views: 15459

f8

NikonUser recommended f8 rather than f32 because at f32 lens diffraction can seriuosly reduce detail. The sweet spot on most lenses is usually the middle stop -- f8 or f11. It will require more shots in the stack, but the detail will be much finer.
by Olympusman
Mon Jan 06, 2014 8:44 am
Forum: Photography Through the Microscope
Topic: Human lung tissue showing cancer cells
Replies: 11
Views: 4055

Cancer cells

While I'm not a pathologist, by comparing myslides of healthy lung tissue which are absent the large bluish masses, I conclude the bluish cell massses are cancerous. I have seen similar cell masses on other histology slides of cancers.
by Olympusman
Sun Jan 05, 2014 9:30 am
Forum: Photography Through the Microscope
Topic: Monkey duodemun (with apologies to Dave Barry)
Replies: 3
Views: 826

Duodenum

Looks like the slide is mislabeled.