Search found 4746 matches

by Pau
Sat Feb 06, 2021 3:49 am
Forum: Equipment Discussions
Topic: Reversing makro-iris Componons
Replies: 11
Views: 396

Re: Reversing makro-iris Componons

Oh man, I'm really sorry to hear that. I'll admit I had only anticipated vertical usage in a microscope on that one--wasn't it for your AZ100? Don't worry, this was entirely my fault, not yours. You're right, the AZ100 was used only vertically, the stupid accident happened when I was dismounting it...
by Pau
Fri Feb 05, 2021 11:51 am
Forum: Equipment Discussions
Topic: Even DF illumination for 4x objective
Replies: 16
Views: 537

Re: Even DF illumination for 4x objective

I have a PC turret condenser, but it does not cover 4X Can you remove the condenser upper lens? With my Zeiss condensers it is needed anyway for 4X and less. Some models allow to switch it out and others to unscrew it. Abbe condensers usually cover the 4X with the upper lens although with some mode...
by Pau
Fri Feb 05, 2021 11:11 am
Forum: Equipment Discussions
Topic: Reversing makro-iris Componons
Replies: 11
Views: 396

Re: Reversing makro-iris Componons

... I haven't tried it on a horizontal setup where stresses would potentially be more severe but the resultant parts are quite solid and tough so I suspect you could design them so that wasn't an issue. Do you remember the nice printed M42 adapter that came with my Nikon V-T tube? it worked well wh...
by Pau
Fri Feb 05, 2021 4:04 am
Forum: Equipment Discussions
Topic: Even DF illumination for 4x objective
Replies: 16
Views: 537

Re: Even DF illumination for 4x objective

DF with a 4X usually is easy, you don't need a dedicated DF condenser if you can place a stop at the right position in an ordinary BF condenser of using a Phase Contrast ring for higher NA objectives as Alan does. Dedicated DF condensers are designed for high NA objectives where DF stops on BF conde...
by Pau
Fri Feb 05, 2021 2:39 am
Forum: Equipment Discussions
Topic: UV light for plants growth
Replies: 7
Views: 311

Re: UV light for plants growth

Thanks for pointing it, if I don't get a reasonable response I'll ban the user
by Pau
Fri Feb 05, 2021 2:19 am
Forum: Equipment Discussions
Topic: UV light for plants growth
Replies: 7
Views: 311

Re: UV light for plants growth

AFAIK, UV is not useful for photosynthesis and it easily photobleaches chlorophyll, even the longer wavelengths.

Because solar radiation includes some UV A (and a little of UV B) plants have some adaptations for its own protection, mainly plants that live under strong solar light.

Any reference?
by Pau
Sat Jan 30, 2021 5:57 am
Forum: Equipment Discussions
Topic: Olympus BH-2 (BHS) LED Conversion
Replies: 5
Views: 519

Re: Olympus BH-2 (BHS) LED Conversion

Time ago I bought one of those retrodiode lamps for my Zeiss microscopes, an older model, the one you link seems clearly improved. Anyway those are my impressions of the one I bought: - powerful enough for most microscope techniques - very good regulation and no flickering if you use the 21KHz PWM (...
by Pau
Wed Jan 27, 2021 1:56 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Auto focussing by moving the camera
Replies: 7
Views: 870

Re: Auto focussing by moving the camera

Some more non ellaborated ideas: Maybe tedious due to the big amount o pictures to check but if the focus difference between stitch positions is not big you could just do a short focus bracketing with a stacking rail at each position and manually select the best frame. Another option could be to use...
by Pau
Sun Jan 24, 2021 10:02 am
Forum: Equipment Discussions
Topic: What the @&$% objective get
Replies: 14
Views: 1031

Re: What the @&$% objective get

JKT wrote:
Sun Jan 24, 2021 3:19 am
An interesting - but mostly academic - question is are they as fragile as the other Mitus? For that price there might be some robustness built-in as well.
Are you suggesting to drop the objective and post the results? :lol: :twisted:
by Pau
Sun Jan 24, 2021 2:26 am
Forum: Equipment Discussions
Topic: Afocal Leica SM-LUX Project
Replies: 19
Views: 2242

Re: Afocal Leica SM-LUX Project

Congrats, it seems to work very well. From what I understand using a camera lens at infinity focus through the intended eyepiece is an easy to do parfocal adaptation that Leitz designed into their microscope systems, designating the phototube and eyepiece with a red dot. Yes, you're right Typical Le...
by Pau
Fri Jan 22, 2021 4:25 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Auto focussing by moving the camera
Replies: 7
Views: 870

Re: Auto focussing by moving the camera

I have dreamed with such a system, both for autofocus and autostacking. :-k Theoretically this could be done if you take the focusing electric signals from the objective bayonet camera mount and feed with them a motor similar to the lens focus one but driving the rail. But usually rail motors are mu...
by Pau
Mon Jan 18, 2021 4:16 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Advice please: High NA air objective with small WD
Replies: 15
Views: 4608

Re: Advice please: High NA air objective with small WD

Question: Do I need to collimate the light source? I was planning to experiment to find out, but you likely know the answer already. As usual in Microscopy the ideal illumination is after Kölher: focusing the light source at the objective rear focal plane filling it. This is what epiilluminators do...
by Pau
Sat Jan 16, 2021 1:16 am
Forum: Equipment Discussions
Topic: 50X objective recommendation
Replies: 31
Views: 3381

Re: 50X objective recommendation

Wow - what kind of rail are you using to reach ~250 nm steps? That is closer to linear servo territory. You can get step sizes almost that size with simple, cheap off-the-shelf microscope focusing block driven by the cheap WeMacro MicroMate. This is what I do, depending of the microscope focus bloc...
by Pau
Sun Jan 10, 2021 6:13 am
Forum: Equipment Discussions
Topic: Monochromatic Microscopes?
Replies: 21
Views: 2595

Re: Monochromatic Microscopes?

I am a little surprised at the low NA. .. ... I am surprised that the NA would go backwards as technology advances. Well, what matters is resolution, not NA itself. The former generation with immersion optics had 1.35 NA and lower resolution, the key as you well know is the shorter wavelength, and ...
by Pau
Fri Jan 08, 2021 11:52 am
Forum: Equipment Discussions
Topic: Raynox dcr150 custom tube lens
Replies: 22
Views: 2842

Re: Raynox dcr150 custom tube lens

Don't forget to put anti-reflection flocking inside to avoid glare