Search found 1309 matches

by enricosavazzi
Sun Oct 11, 2020 7:28 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Effective aperture with teleconverter + macro lens
Replies: 6
Views: 2105

Effective aperture with teleconverter + macro lens

Let's see whether I understand the problem correctly, or I am making a mistake somewhere. We assume that the pupil ratio is 1 for simplicity, so the effective aperture A' is A' = A * (m + 1) where A is the nominal aperture, and m the magnification. So if we focus a lens at m = 2 by extension alone (...
by enricosavazzi
Fri Oct 09, 2020 9:37 am
Forum: Equipment Discussions
Topic: Olympus BX stage keeps drifting down, "focus shifting".
Replies: 20
Views: 3456

Re: Olympus BX stage keeps drifting down, "focus shifting".

[...] I am using BX53 stand just to clarify, very interesting pointers about provis models, well I really like 53 because it has small footprint and does not take much space on my very busy table! [...] Indeed, the Provis stand weighs north of 50 kg "naked", needs a strong table, and most functions...
by enricosavazzi
Thu Oct 08, 2020 12:02 pm
Forum: Equipment Discussions
Topic: Olympus BX stage keeps drifting down, "focus shifting".
Replies: 20
Views: 3456

Re: Olympus BX stage keeps drifting down, "focus shifting".

The following information does not help if you already have a BX series microscope, but it is related to thermal drift so it might be interesting nonetheless. A couple of years ago, I ended up ordering an Olympus AX70 stand. It ended up being lost in the mail and eventually returned to the sender, s...
by enricosavazzi
Sun Oct 04, 2020 2:56 am
Forum: Equipment Discussions
Topic: Olympus BX stage keeps drifting down, "focus shifting".
Replies: 20
Views: 3456

Re: Olympus BX stage keeps drifting down.

Just in case, check the friction/brake on the focusing rack before you do any maintenance. This lever may be set too loose. The lever that controls friction is located between the left coarse focus knob and the stand.
by enricosavazzi
Sat Oct 03, 2020 2:01 am
Forum: Equipment Discussions
Topic: DIY Fluorescence with Zeiss fluoro condenser - help needed
Replies: 75
Views: 14189

Re: DIY Fluorescence with Zeiss fluoro condenser - help needed

This thread is already quite long, so I might be repeating some information already state above. However, here it goes: Non-stained acrylic (=Plexiglas and other names in commercial nomenclature) transmits UV well into the UVB range. Many Acrylic formulations have UV-absorbing dyes mixed in, but not...
by enricosavazzi
Tue Sep 22, 2020 12:01 am
Forum: Equipment Discussions
Topic: UV against lens fungus
Replies: 3
Views: 1389

Re: UV against lens fungus

Can UV light stop fungus growth inside objective ? The most effective wavelengths for this use are in the UVB and UVC. Most lenses (except the very few ones designed for UV imaging and a handful more) transmit little to no UVA, and no UVB and UVC, so exposing these lenses to UVB and UVC has no effe...
by enricosavazzi
Sun Sep 13, 2020 2:10 am
Forum: Equipment Discussions
Topic: Question about window on the underside of Olympus trinocular head
Replies: 25
Views: 5575

Re: Question about window on the underside of Olympus trinocular head

In a worst-case scenario, the window may have been computed as part of the optical system w.r.t. axial chromatic aberration, and removing it may increase said aberration. I have seen several examples of optical windows in Olympus infinite microscopes where a thick optical window has a thin cladding ...
by enricosavazzi
Fri Sep 11, 2020 12:09 am
Forum: Macro & Microscopy Articles
Topic: Anesthesia, analgesia, and euthanasia of invertebrates
Replies: 0
Views: 1927

Anesthesia, analgesia, and euthanasia of invertebrates

Cooper 2011 - Anesthesia, analgesia, and euthanasia of invertebrates. ILAR Journal 52 (2): 196-204

https://academic.oup.com/ilarjournal/ar ... 52-196.pdf
by enricosavazzi
Tue Sep 08, 2020 3:57 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Tranforming diatoms into magnesium replicas
Replies: 5
Views: 1965

Re: Tranforming diatoms into magnesium replicas

bralex wrote:
Tue Sep 08, 2020 3:41 pm
I think he's saying "magnesia" not "magnesium" when he talks about the replica.
That would explain it. Magnesia is magnesium oxide, so he may have said the right thing after all.
by enricosavazzi
Tue Sep 08, 2020 12:32 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Tranforming diatoms into magnesium replicas
Replies: 5
Views: 1965

Re: Tranforming diatoms into magnesium replicas

The speaker does say "magnesium replica", but the formula shown in the presentation seems to say that the replica material is magnesium oxide . While metallic magnesium is opaque to VIS (albeit very reflective), magnesium oxide is trasparent from NUV to IR, see https://www.osapublishing.org/josa/abs...
by enricosavazzi
Tue Aug 25, 2020 10:20 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Questions for the experts.
Replies: 12
Views: 2612

Re: Questions for the experts.

I will leave some answers to others, but can say that vibration in your setup seems to take an excessively long time to settle at 10x, so you should try to solve this problem. For example, mounting the XY stage atop the scissor jack is probably not the best idea. I would try to reverse the order and...
by enricosavazzi
Mon Aug 17, 2020 5:01 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Would you recommend a tripod or monopod for macro in the field?
Replies: 23
Views: 6492

Re: Would you recommend a tripod or monopod for macro in the field?

Hi, Thanks everyone for the suggestions. @Dalantech That's a great idea. It doesn't lend itself to my usual setup with a 105 mm lens (my hands aren't that big :wink: ) but I recently started using the EF-S 24mm for macro, which would be suitable. Still have to figure out if I need to drink more or ...
by enricosavazzi
Fri Aug 14, 2020 5:12 am
Forum: Equipment Discussions
Topic: Help needed: DIC components necessary for my Oly
Replies: 31
Views: 6229

Re: Help needed: DIC components necessary for my Oly

First of all, in my experience the UIS DIC system is very sensitive to mismatches between objective and DIC prism. For example, on my system the UDP10 prism works well with the UPlanApo 10x 0.40 (from the UIS series) and poorly with the UPlanFl N 10x 0.30 (from the UIS2 series). My recommendation fo...
by enricosavazzi
Thu Aug 13, 2020 11:03 pm
Forum: Equipment Discussions
Topic: The Canon R5
Replies: 20
Views: 5084

Re: The Canon R5

[...] I quick thought, how about making sure the fan has literally no contact to the heatsink, while also being as closely placed to it as possible? This could work out in our favour. One possible problem is that turbulence created by the fan blades in the air flow can transmit vibrations to the he...
by enricosavazzi
Thu Aug 06, 2020 1:02 am
Forum: Equipment Discussions
Topic: Sony A7R with microscope lens
Replies: 32
Views: 8608

Re: Sony A7R with microscope lens

Metabones seems to no longer produce these adapters (I guess they discovered that they can earn more from "smart" Canon to Sony adapters and speed boosters). However, second-hand Metabones "dumb" adapters can still be found on the second-hand market, sometimes at relatively good prices. B&H has thi...