As Pau said, thank you very much Johny.

Do you have optics beside a mirror in your 3-way Cube? I am thinking about building a similar thing to mount an HBO and a LED at the same time. It would be nice to switch them without much mechanical work. Any would be appreciated.

Kind regards
Peter

Hi, there is a paper out detailing a LED illumination axis made with simple off the shelf components (Arduino, Thorlabs, etc.). They also have a comparision of critical and Koehler illumination: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143547 Edit: I think besides having le...

Hi, I also found the centering procedure outlined in the olympus RFA manual produced sufficiently good results for me. Olympus advises the use of a centering target (U-CST) which I don't have and always replaced by a sheet of cardboard with a cross drawn on it or just by a plain sheet of paper/cardb...

Jacek! Thank you for the info.
What Diodes do you use? Are you willing to share the information.

Your new pictures are stunning (and it is somewhat comforting to see that I am not the only one having problems with stray highly fluorescent fibres).

Peter

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter

Anne - why the question marks?

I think your microphotographs have a very distinct style - both in the technical execution and in the framing of the subjects. 'Never inteded to say anything else :-)

Peter

Anne,
thank you for these nice images. Your photography is - as always - very precise and to the point. I enjoy this very much.

Kind regards
Peter

I also enjoy that you include scalebars for your images!

Hi, i am toying around with my new UV excitation autofluorescence setup and took some garden variety images. Twig of a privet hedge (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_lingu2_1.jpg Ghostberry (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_ghostberry_1.jpg...

Seems like a reasonable explanation. Thank you Enrico and zzffnn!

Do you by chance know why Olympus has a line of non "U" Apo objectives (e.g. the "PlanApo 60 1.40").

Hi, recently I came across an unusual 10x Olympus objective: A UPLanApo 10x Oil NA 0.40 and thought that it might be of interest to some members of the Forum. http://www.photomacrography.net/forum/userpix/6661_10xOil_1.jpg Oil immersion in a 10x is uncommon (to the point that I have never seen one) ...

George, Chris, thank you for your kind words. @ Chris DAPI and fading is a non-issue for me. I am not sure if you mean fading over time (storage) or photobleaching during illumination. The DAPI signal stays stable over months even at room temperature and i am sure that it will last even longer. Duri...

Hi, micro manager can directly interface with a wide variety of stages and other peripherals. See section Stages, filter wheels, shutters of the Micro Manager device support (1) for details. These are however mostly very expensive but from time to time and with patience you may get lucky on Ebay. I ...

Also some examples from recent work. This is more on the work than on the hobby side - but nevertheless taken on my setup: UPlanFl 60XOI @ NA 1.25 with HR-DIC on a dry NA 0.9 condenser taken with a Point Grey GS2 monochrome camera in Micro-Manager. The image is bronchial epithelium with a dual immun...

Dear photomacrography and amateurmicrography community, after a sometime reading and a shorter time posting I feel that it is my time to introduce myself. I am working in pathology and by this am able to enjoy microscopy most of my working day - I hope this does not disqualify me from the amateurmic...