As Pau said, thank you very much Johny.
Do you have optics beside a mirror in your 3-way Cube? I am thinking about building a similar thing to mount an HBO and a LED at the same time. It would be nice to switch them without much mechanical work. Any would be appreciated.
Kind regards
Peter
Search found 81 matches
- Wed Jan 16, 2019 9:25 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 11375
- Wed Jan 16, 2019 5:11 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 11375
Hi, there is a paper out detailing a LED illumination axis made with simple off the shelf components (Arduino, Thorlabs, etc.). They also have a comparision of critical and Koehler illumination: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143547 Edit: I think besides having le...
- Mon Jan 14, 2019 1:52 am
- Forum: Equipment Discussions
- Topic: "Special objective" for centering epi illuminators
- Replies: 6
- Views: 2939
Hi, I also found the centering procedure outlined in the olympus RFA manual produced sufficiently good results for me. Olympus advises the use of a centering target (U-CST) which I don't have and always replaced by a sheet of cardboard with a cross drawn on it or just by a plain sheet of paper/cardb...
- Fri Jan 11, 2019 1:14 am
- Forum: Photography Through the Microscope
- Topic: Various
- Replies: 22
- Views: 6093
- Thu Jan 10, 2019 12:40 pm
- Forum: Photography Through the Microscope
- Topic: Various
- Replies: 22
- Views: 6093
- Mon Dec 31, 2018 4:44 am
- Forum: Photography Through the Microscope
- Topic: Diatoms from Barbados
- Replies: 13
- Views: 2751
- Sun Dec 30, 2018 7:44 am
- Forum: Photography Through the Microscope
- Topic: Diatoms from Barbados
- Replies: 13
- Views: 2751
- Sat Dec 29, 2018 6:31 am
- Forum: Photography Through the Microscope
- Topic: Garden variety autofluorescence
- Replies: 1
- Views: 1277
Garden variety autofluorescence
Hi, i am toying around with my new UV excitation autofluorescence setup and took some garden variety images. Twig of a privet hedge (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_lingu2_1.jpg Ghostberry (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_ghostberry_1.jpg...
- Fri Dec 21, 2018 1:40 am
- Forum: Equipment Discussions
- Topic: Unusual Olympus 10x
- Replies: 9
- Views: 3640
- Wed Dec 19, 2018 2:27 pm
- Forum: Equipment Discussions
- Topic: Unusual Olympus 10x
- Replies: 9
- Views: 3640
- Tue Dec 18, 2018 11:44 am
- Forum: Equipment Discussions
- Topic: Unusual Olympus 10x
- Replies: 9
- Views: 3640
Unusual Olympus 10x
Hi, recently I came across an unusual 10x Olympus objective: A UPLanApo 10x Oil NA 0.40 and thought that it might be of interest to some members of the Forum. http://www.photomacrography.net/forum/userpix/6661_10xOil_1.jpg Oil immersion in a 10x is uncommon (to the point that I have never seen one) ...
- Fri Dec 14, 2018 10:08 am
- Forum: Community Members and Friends
- Topic: Hi from northern Germany
- Replies: 5
- Views: 6964
George, Chris, thank you for your kind words. @ Chris DAPI and fading is a non-issue for me. I am not sure if you mean fading over time (storage) or photobleaching during illumination. The DAPI signal stays stable over months even at room temperature and i am sure that it will last even longer. Duri...
- Thu Dec 13, 2018 12:47 pm
- Forum: Equipment Discussions
- Topic: Anatomy, A DIY Precision S & S System (First Light)
- Replies: 27
- Views: 7835
Hi, micro manager can directly interface with a wide variety of stages and other peripherals. See section Stages, filter wheels, shutters of the Micro Manager device support (1) for details. These are however mostly very expensive but from time to time and with patience you may get lucky on Ebay. I ...
- Thu Dec 13, 2018 4:17 am
- Forum: Community Members and Friends
- Topic: Hi from northern Germany
- Replies: 5
- Views: 6964
Also some examples from recent work. This is more on the work than on the hobby side - but nevertheless taken on my setup: UPlanFl 60XOI @ NA 1.25 with HR-DIC on a dry NA 0.9 condenser taken with a Point Grey GS2 monochrome camera in Micro-Manager. The image is bronchial epithelium with a dual immun...
- Thu Dec 13, 2018 4:10 am
- Forum: Community Members and Friends
- Topic: Hi from northern Germany
- Replies: 5
- Views: 6964
Hi from northern Germany
Dear photomacrography and amateurmicrography community, after a sometime reading and a shorter time posting I feel that it is my time to introduce myself. I am working in pathology and by this am able to enjoy microscopy most of my working day - I hope this does not disqualify me from the amateurmic...