Search found 77 matches

by pbraub
Thu Jan 10, 2019 12:40 pm
Forum: Photography Through the Microscope
Topic: Various
Replies: 22
Views: 5962

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter
by pbraub
Mon Dec 31, 2018 4:44 am
Forum: Photography Through the Microscope
Topic: Diatoms from Barbados
Replies: 13
Views: 2661

Anne - why the question marks?

I think your microphotographs have a very distinct style - both in the technical execution and in the framing of the subjects. 'Never inteded to say anything else :-)

Peter
by pbraub
Sun Dec 30, 2018 7:44 am
Forum: Photography Through the Microscope
Topic: Diatoms from Barbados
Replies: 13
Views: 2661

Anne,
thank you for these nice images. Your photography is - as always - very precise and to the point. I enjoy this very much.

Kind regards
Peter

I also enjoy that you include scalebars for your images!
by pbraub
Sat Dec 29, 2018 6:31 am
Forum: Photography Through the Microscope
Topic: Garden variety autofluorescence
Replies: 1
Views: 1272

Garden variety autofluorescence

Hi, i am toying around with my new UV excitation autofluorescence setup and took some garden variety images. Twig of a privet hedge (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_lingu2_1.jpg Ghostberry (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_ghostberry_1.jpg...
by pbraub
Fri Dec 21, 2018 1:40 am
Forum: Equipment Discussions
Topic: Unusual Olympus 10x
Replies: 9
Views: 3589

Seems like a reasonable explanation. Thank you Enrico and zzffnn!
by pbraub
Wed Dec 19, 2018 2:27 pm
Forum: Equipment Discussions
Topic: Unusual Olympus 10x
Replies: 9
Views: 3589

Do you by chance know why Olympus has a line of non "U" Apo objectives (e.g. the "PlanApo 60 1.40").
by pbraub
Tue Dec 18, 2018 11:44 am
Forum: Equipment Discussions
Topic: Unusual Olympus 10x
Replies: 9
Views: 3589

Unusual Olympus 10x

Hi, recently I came across an unusual 10x Olympus objective: A UPLanApo 10x Oil NA 0.40 and thought that it might be of interest to some members of the Forum. http://www.photomacrography.net/forum/userpix/6661_10xOil_1.jpg Oil immersion in a 10x is uncommon (to the point that I have never seen one) ...
by pbraub
Fri Dec 14, 2018 10:08 am
Forum: Community Members and Friends
Topic: Hi from northern Germany
Replies: 5
Views: 6222

George, Chris, thank you for your kind words. @ Chris DAPI and fading is a non-issue for me. I am not sure if you mean fading over time (storage) or photobleaching during illumination. The DAPI signal stays stable over months even at room temperature and i am sure that it will last even longer. Duri...
by pbraub
Thu Dec 13, 2018 12:47 pm
Forum: Equipment Discussions
Topic: Anatomy, A DIY Precision S & S System (First Light)
Replies: 27
Views: 7735

Hi, micro manager can directly interface with a wide variety of stages and other peripherals. See section Stages, filter wheels, shutters of the Micro Manager device support (1) for details. These are however mostly very expensive but from time to time and with patience you may get lucky on Ebay. I ...
by pbraub
Thu Dec 13, 2018 4:17 am
Forum: Community Members and Friends
Topic: Hi from northern Germany
Replies: 5
Views: 6222

Also some examples from recent work. This is more on the work than on the hobby side - but nevertheless taken on my setup: UPlanFl 60XOI @ NA 1.25 with HR-DIC on a dry NA 0.9 condenser taken with a Point Grey GS2 monochrome camera in Micro-Manager. The image is bronchial epithelium with a dual immun...
by pbraub
Thu Dec 13, 2018 4:10 am
Forum: Community Members and Friends
Topic: Hi from northern Germany
Replies: 5
Views: 6222

Hi from northern Germany

Dear photomacrography and amateurmicrography community, after a sometime reading and a shorter time posting I feel that it is my time to introduce myself. I am working in pathology and by this am able to enjoy microscopy most of my working day - I hope this does not disqualify me from the amateurmic...
by pbraub
Wed Dec 12, 2018 12:05 am
Forum: Equipment Discussions
Topic: Anatomy, A DIY Precision S & S System (First Light)
Replies: 27
Views: 7735

Hi Mike, thank you for your detailed report on developing and building your system. I really enjoy reading along. Have you tried ImageJ for stitching your images (especially the Grid/Collection stitching plugin). I have successfully stitched microscopy image up to 10 GB on my relatively modest machi...
by pbraub
Mon Dec 10, 2018 11:55 am
Forum: Macro & Microscopy Articles
Topic: Blog about optical and electron microscopy
Replies: 8
Views: 8710

Thank you! I have read your article on superresolution with the Zeiss universal and was very, very impressed. Superresolution in the home-shop (this is the right kind of crazy!). Thank you for sharing the details on your blog. Have you ever tried different excitation wavelengths with your setup? Do ...
by pbraub
Thu Dec 06, 2018 12:22 pm
Forum: Equipment Discussions
Topic: tube lens comparison
Replies: 17
Views: 3866

Thank you for your descriptions and sharing the photos!
I did not expect it to be so complex, but in the end I did not know what to expect. I think this will make an interesting project for you.

Peter
by pbraub
Thu Nov 29, 2018 12:51 pm
Forum: Equipment Discussions
Topic: tube lens comparison
Replies: 17
Views: 3866

The U-Photo is a monster. Can you share your experiences? I have a SWTR30 with SWHK eyepieces, but it is currently fitted to my work scope and will remain there for the foreseeable future (I enjoy the 26.5 image circle). I cannot easily use it in my test setups. Has somebody noticed any differences ...