Do you have the option to use an higher NA objective to establish a ground truth?
Similar to this approach in microscopy (see figs 1 and 2)?
Peter
Search found 81 matches
- Mon Mar 04, 2019 1:02 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Trying to understand Topaz Sharpen AI
- Replies: 7
- Views: 3410
- Sat Mar 02, 2019 1:22 pm
- Forum: Photography Through the Microscope
- Topic: Human cerebellum
- Replies: 3
- Views: 1370
Thank you for these nice pictures. I am not sure, but the first three pictures seem to show the outer (molecular) layer of the cerebellum which has many argyrophile fibers and fewer cells. The last 3 pictures show Purkinje Cells sitting at the border of the nuclear (inner) layer and the outer molecu...
- Fri Feb 15, 2019 5:59 am
- Forum: Equipment Discussions
- Topic: Barrier filter for autofluorescence
- Replies: 6
- Views: 2891
Well, I'd guess a longpass with appropriate cut-on frequency (it is the widest spectrum you can get in terms of emission). However they write on their hompage "... We employ a technique that we call “wide-spectrum fluorescence”..." and I am not 100% sure that they speak only about the barrier / emis...
- Fri Feb 15, 2019 3:22 am
- Forum: Equipment Discussions
- Topic: Barrier filter for autofluorescence
- Replies: 6
- Views: 2891
- Fri Feb 15, 2019 1:00 am
- Forum: Equipment Discussions
- Topic: What is the "Umbra shield" ?
- Replies: 4
- Views: 2779
- Fri Feb 15, 2019 12:50 am
- Forum: Equipment Discussions
- Topic: Filters for Olympus CX41 (phase, polarization and darkfield)
- Replies: 21
- Views: 6033
Hi, Alan Wood is correct. The CX-PH1,2,3 clicks into the CX condenser like the CX-AL (aux lens for centering, the condenser itself cannot be centered). For darkfield and oblique (at least) one cheap option to try is to create a DIY stop out of black cardboard and put this below the condenser (or san...
- Fri Feb 15, 2019 12:36 am
- Forum: Photography Through the Microscope
- Topic: Pinus sylvestris autofluorescence stack'n stich
- Replies: 9
- Views: 2037
- Fri Feb 15, 2019 12:33 am
- Forum: Equipment Discussions
- Topic: Barrier filter for autofluorescence
- Replies: 6
- Views: 2891
I would rather ask: what color/wavelength for excitation do you want to use? If you want UV excitation @365nm for example you could choose an appropriate longpass filter (400 nm LP for example) . Then you can detect all the higher wavelengths (all the colors). Depending on the quality of your lights...
- Wed Feb 13, 2019 11:56 am
- Forum: Equipment Discussions
- Topic: Rate these Camera adapters for Olympus CX41
- Replies: 17
- Views: 6856
The A7II should support live view with capture one pro. I think there is a pro sony version at a reduced price. The for-sony free version does not support live view AFAIK. https://www.phaseone.com/capture-one/supported-cameras.aspx?c=sony Please share your experiences. This might be a way I would li...
- Tue Feb 12, 2019 8:30 am
- Forum: Equipment Discussions
- Topic: Rate these Camera adapters for Olympus CX41
- Replies: 17
- Views: 6856
I just swapped the superwide tube (SWHK) to the CX41 (what a strange sight) and it delivered a nice and even picture on a Plan 40x (!). So I do not think that the impact of the small dovetail airspace is very large. It might well depend on your preferences and whether or not you aim to push everythi...
- Tue Feb 12, 2019 12:44 am
- Forum: Equipment Discussions
- Topic: Rate these Camera adapters for Olympus CX41
- Replies: 17
- Views: 6856
One difference between the CX and the BX scopes is that the cental opening in the dovetail is smaller in CX. I don't know if this will be relevant for example in terms of vignetting when projecting to larger sensors. The CX tubes and oculars have a FN of 20 but the scope works well with the BX FN22 ...
- Sun Feb 10, 2019 2:14 am
- Forum: Equipment Discussions
- Topic: Laser or Waterjet cut metal parts (Europe)
- Replies: 2
- Views: 2649
Laser or Waterjet cut metal parts (Europe)
Hi, has anyone of you good experience with a workshop producing custom laser or waterjet cut metal parts (aluminium or steel). Preferably located in Europe to reduce shipping costs. I have seen several shops while doing google searches but would be glad if there anyone of you could recommend a tried...
- Thu Jan 24, 2019 1:24 am
- Forum: Equipment Discussions
- Topic: Oil immersion condenser
- Replies: 25
- Views: 8178
Hi, maybe this is of relevance within this discussion: I have read about a clever way to higher NA illumination (than observation) for superresolution. There is a paper out which does this with a off the shelf LCD as light modulator in the condenser back focal plane. I would like to replicate this s...
- Wed Jan 23, 2019 6:30 am
- Forum: Equipment Discussions
- Topic: THK KR15 cheap on eBay
- Replies: 78
- Views: 21256
- Tue Jan 22, 2019 11:37 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 11356
The HBO Lamps need some time to stabilize after ignition (15-20 min), this is especially relevant if you want to take stacks. Do not turn off immediately after ignition and do not re-ignite a hot lamp. Both shortens the lifespan. The olympus BX-RFA manual also says #To extend the mercury burner life...