Search found 81 matches

by pbraub
Mon Mar 04, 2019 1:02 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Trying to understand Topaz Sharpen AI
Replies: 7
Views: 3410

Do you have the option to use an higher NA objective to establish a ground truth?

Similar to this approach in microscopy (see figs 1 and 2)?

Peter
by pbraub
Sat Mar 02, 2019 1:22 pm
Forum: Photography Through the Microscope
Topic: Human cerebellum
Replies: 3
Views: 1370

Thank you for these nice pictures. I am not sure, but the first three pictures seem to show the outer (molecular) layer of the cerebellum which has many argyrophile fibers and fewer cells. The last 3 pictures show Purkinje Cells sitting at the border of the nuclear (inner) layer and the outer molecu...
by pbraub
Fri Feb 15, 2019 5:59 am
Forum: Equipment Discussions
Topic: Barrier filter for autofluorescence
Replies: 6
Views: 2891

Well, I'd guess a longpass with appropriate cut-on frequency (it is the widest spectrum you can get in terms of emission). However they write on their hompage "... We employ a technique that we call “wide-spectrum fluorescence”..." and I am not 100% sure that they speak only about the barrier / emis...
by pbraub
Fri Feb 15, 2019 3:22 am
Forum: Equipment Discussions
Topic: Barrier filter for autofluorescence
Replies: 6
Views: 2891

@Pau
I was indeed referring to epifluorescence. Wouldnt you use a similar setup for "macro": Excitation filter on the UV Flashlight (for example) to cut any wavelengths higher than necessary and a longpass barrier filter. At least this would have been my approach.
by pbraub
Fri Feb 15, 2019 1:00 am
Forum: Equipment Discussions
Topic: What is the "Umbra shield" ?
Replies: 4
Views: 2779

Or you could turn down the lights in your room...

This however is often not possible in multi-user labspaces. The CKX is often located in normal cell culture labs for quick inspection of cultures and not in dedicated microscope rooms for measurement which are usually dark.
by pbraub
Fri Feb 15, 2019 12:50 am
Forum: Equipment Discussions
Topic: Filters for Olympus CX41 (phase, polarization and darkfield)
Replies: 21
Views: 6033

Hi, Alan Wood is correct. The CX-PH1,2,3 clicks into the CX condenser like the CX-AL (aux lens for centering, the condenser itself cannot be centered). For darkfield and oblique (at least) one cheap option to try is to create a DIY stop out of black cardboard and put this below the condenser (or san...
by pbraub
Fri Feb 15, 2019 12:36 am
Forum: Photography Through the Microscope
Topic: Pinus sylvestris autofluorescence stack'n stich
Replies: 9
Views: 2037

This is a very nice image. Thank you.

How did you prepare the sample?
by pbraub
Fri Feb 15, 2019 12:33 am
Forum: Equipment Discussions
Topic: Barrier filter for autofluorescence
Replies: 6
Views: 2891

I would rather ask: what color/wavelength for excitation do you want to use? If you want UV excitation @365nm for example you could choose an appropriate longpass filter (400 nm LP for example) . Then you can detect all the higher wavelengths (all the colors). Depending on the quality of your lights...
by pbraub
Wed Feb 13, 2019 11:56 am
Forum: Equipment Discussions
Topic: Rate these Camera adapters for Olympus CX41
Replies: 17
Views: 6856

The A7II should support live view with capture one pro. I think there is a pro sony version at a reduced price. The for-sony free version does not support live view AFAIK. https://www.phaseone.com/capture-one/supported-cameras.aspx?c=sony Please share your experiences. This might be a way I would li...
by pbraub
Tue Feb 12, 2019 8:30 am
Forum: Equipment Discussions
Topic: Rate these Camera adapters for Olympus CX41
Replies: 17
Views: 6856

I just swapped the superwide tube (SWHK) to the CX41 (what a strange sight) and it delivered a nice and even picture on a Plan 40x (!). So I do not think that the impact of the small dovetail airspace is very large. It might well depend on your preferences and whether or not you aim to push everythi...
by pbraub
Tue Feb 12, 2019 12:44 am
Forum: Equipment Discussions
Topic: Rate these Camera adapters for Olympus CX41
Replies: 17
Views: 6856

One difference between the CX and the BX scopes is that the cental opening in the dovetail is smaller in CX. I don't know if this will be relevant for example in terms of vignetting when projecting to larger sensors. The CX tubes and oculars have a FN of 20 but the scope works well with the BX FN22 ...
by pbraub
Sun Feb 10, 2019 2:14 am
Forum: Equipment Discussions
Topic: Laser or Waterjet cut metal parts (Europe)
Replies: 2
Views: 2649

Laser or Waterjet cut metal parts (Europe)

Hi, has anyone of you good experience with a workshop producing custom laser or waterjet cut metal parts (aluminium or steel). Preferably located in Europe to reduce shipping costs. I have seen several shops while doing google searches but would be glad if there anyone of you could recommend a tried...
by pbraub
Thu Jan 24, 2019 1:24 am
Forum: Equipment Discussions
Topic: Oil immersion condenser
Replies: 25
Views: 8178

Hi, maybe this is of relevance within this discussion: I have read about a clever way to higher NA illumination (than observation) for superresolution. There is a paper out which does this with a off the shelf LCD as light modulator in the condenser back focal plane. I would like to replicate this s...
by pbraub
Wed Jan 23, 2019 6:30 am
Forum: Equipment Discussions
Topic: THK KR15 cheap on eBay
Replies: 78
Views: 21256

Viktor,

thank you for keeping the monologue going. These are the kind of threads I often refer to when planning a project such as yours.

Kind regards
Peter
by pbraub
Tue Jan 22, 2019 11:37 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 11356

The HBO Lamps need some time to stabilize after ignition (15-20 min), this is especially relevant if you want to take stacks. Do not turn off immediately after ignition and do not re-ignite a hot lamp. Both shortens the lifespan. The olympus BX-RFA manual also says #To extend the mercury burner life...