Search found 77 matches

by pbraub
Tue May 28, 2019 5:46 am
Forum: Equipment Discussions
Topic: Use cases for ELWD Condensers
Replies: 8
Views: 1845

Hi, Pau is right. The ELWD condensers are used on inverted microscopes. In the lab I work in we use a NA 0.3 elwd condenser with phase contrast on a small scope for checking cell cultures (Olympus CKX41) (this is commonly right next to the incubator in the lab for visual inspection - vitalitiy, cont...
by pbraub
Fri May 17, 2019 8:40 am
Forum: Equipment Exchange
Topic: Shellac ringing cement
Replies: 2
Views: 2169

You can also use (clear) nail varnish. However, it does not look as fancy as ringing cement.

Peter
by pbraub
Mon Apr 29, 2019 1:03 am
Forum: Equipment Exchange
Topic: Olympus condenser DF/Pol/Rheinberg/Oblique insert set
Replies: 7
Views: 2241

This is an interesting piece of equipent! Does this also fit the Swing-Out type condenser (U-SC?)

Kind regards
Peter
by pbraub
Sat Apr 27, 2019 12:50 pm
Forum: Photography Through the Microscope
Topic: Scalariform conjugation in Spirogyra
Replies: 1
Views: 705

Thank you for these nice pictures. I like the last one best - it is so calm and reduced to the essentials. It nearly looks like it could be tiled to infinity.

Peter
by pbraub
Mon Apr 08, 2019 7:28 am
Forum: Photography Through the Microscope
Topic: Various algaes
Replies: 13
Views: 1930

Anne,

I really like the crisp and clean look of your images. Fantastic. Thank you.

However no scalebar this time :-( - i suppose for the sake of art.

Peter
by pbraub
Sun Apr 07, 2019 11:42 pm
Forum: Photography Through the Microscope
Topic: VIS vs FL
Replies: 10
Views: 1593

Mazek,

thank you for the - again - wonderful photos and for the information on your setup!

Cheers
Peter
by pbraub
Sat Apr 06, 2019 11:58 am
Forum: Photography Through the Microscope
Topic: VIS vs FL
Replies: 10
Views: 1593

Maszek, thank you for these impressive images - I can even get the stereo images to fuse in my head (which I have some troubles with usually).

Is the FL pure UV excitation or do you use multiple wavelengths?

Cheers
Peter
by pbraub
Wed Apr 03, 2019 10:33 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: More mass is better right?
Replies: 7
Views: 3599

I know. Thorlabs even advertises their composite breadboards with a technical note stating that more weight is not necessarily better.

However, the thought of doing macro on a running mill makes me smile. I can't help it.
by pbraub
Wed Apr 03, 2019 8:28 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: More mass is better right?
Replies: 7
Views: 3599

ray_parkhurst wrote:Are your vibrations gone?
With the mill on or off that is?
by pbraub
Fri Mar 29, 2019 9:01 am
Forum: Photography Through the Microscope
Topic: Dog Urinary Bladder
Replies: 3
Views: 1205

Bill is rigth, this is definitely gall and not urinary bladder.

In Bills picture the mucosa is expanded by foamy cells (macrophages) under the epithelum. This is called "cholestatosis". Olympusmans pictures show gallbladder without any significant pathological changes.

Peter
by pbraub
Sun Mar 24, 2019 12:59 pm
Forum: Photography Through the Microscope
Topic: Lymph node showing cancer cells
Replies: 3
Views: 962

The small dark cells are lymphocytes of the orginial lymphnote. They form swirling pattern around a germinal center. The cancer cells are the ones with a lighter red-ish color. Note how much more irregular the nuclei are. The lymphocyte nuclei are pretty much uniform where the neoplastic cells have ...
by pbraub
Mon Mar 04, 2019 1:02 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Trying to understand Topaz Sharpen AI
Replies: 7
Views: 3293

Do you have the option to use an higher NA objective to establish a ground truth?

Similar to this approach in microscopy (see figs 1 and 2)?

Peter
by pbraub
Sat Mar 02, 2019 1:22 pm
Forum: Photography Through the Microscope
Topic: Human cerebellum
Replies: 3
Views: 1368

Thank you for these nice pictures. I am not sure, but the first three pictures seem to show the outer (molecular) layer of the cerebellum which has many argyrophile fibers and fewer cells. The last 3 pictures show Purkinje Cells sitting at the border of the nuclear (inner) layer and the outer molecu...
by pbraub
Fri Feb 15, 2019 5:59 am
Forum: Equipment Discussions
Topic: Barrier filter for autofluorescence
Replies: 6
Views: 2869

Well, I'd guess a longpass with appropriate cut-on frequency (it is the widest spectrum you can get in terms of emission). However they write on their hompage "... We employ a technique that we call “wide-spectrum fluorescence”..." and I am not 100% sure that they speak only about the barrier / emis...
by pbraub
Fri Feb 15, 2019 3:22 am
Forum: Equipment Discussions
Topic: Barrier filter for autofluorescence
Replies: 6
Views: 2869

@Pau
I was indeed referring to epifluorescence. Wouldnt you use a similar setup for "macro": Excitation filter on the UV Flashlight (for example) to cut any wavelengths higher than necessary and a longpass barrier filter. At least this would have been my approach.