Search found 465 matches

by JohnyM
Thu Jan 24, 2019 5:58 am
Forum: Equipment Discussions
Topic: Oil immersion condenser
Replies: 25
Views: 7732

Dic prisms are magnification dependant. Oil / dry makes no difference to them.
Ie: i have 1.4 oil DIC condenser with oil dic prisms that work just as well with top lens oiled or dy.
But when i change top lens to 0.9 prisms stop prodicing usable DIC image. I need to change prisms as well.
by JohnyM
Wed Jan 23, 2019 5:04 pm
Forum: Equipment Discussions
Topic: Oil immersion condenser
Replies: 25
Views: 7732

It's not a big secret, that air immersed glass is limited to 0.95
Abbe already knew that.
by JohnyM
Wed Jan 23, 2019 8:47 am
Forum: Equipment Discussions
Topic: Oil immersion condenser
Replies: 25
Views: 7732

Resolution equation consist of:
Wavelenght / objective NA + condenser NA

So when you need to achive higher resolution, you oil your condenser.
Condenser NA cannot exceed Objective NA.
by JohnyM
Tue Jan 22, 2019 8:02 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

All my cubes are Nikon original and look like the right one. I know nothing about the left one. I had 4 different versions of that "4mm slider". One was empty, one had polarizer, one had auxiliary barier filter and one was blocking the light. Auxilary barier filters are actually usefull to tune the ...
by JohnyM
Tue Jan 22, 2019 6:35 am
Forum: Equipment Discussions
Topic: Judging high-NA infinity-corrected objectives
Replies: 62
Views: 17404

In the very near future I will be able to compare the Nikon 20/0.75 with a 2X magnification changer against a native 40/0.75 I do have Microphot with mag changer and some same Na different mag objectives (Finite CFN). 20x/0,75 is effectively replacing 40x/0.75 in my setup (10x/0,45 ; 20x/0,75 ; 40x...
by JohnyM
Fri Jan 18, 2019 7:30 am
Forum: Equipment Discussions
Topic: THK KR15 cheap on eBay
Replies: 78
Views: 20936

Well, my pair will (hopefully) turn into microscope stage :) I find 30mm travel too small for anything else than micro (mine starts from 10x) JohnyM...same question for you...any update? ...Ray Attempt to adapt them to microscope stage proved to be a disaster. Instead i moved them to XYZ horizontal...
by JohnyM
Wed Jan 16, 2019 8:32 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

I was suprized myself. On the other hand it's mirrored 100W vs 3W. Anyway, those LED's are fishy anyway. They look all good on paper, but 365 is leaking violet and supposed to be blue is shining through GIF.
by JohnyM
Wed Jan 16, 2019 6:31 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

This is flash test in UV. http://www.photomacrography.net/forum/userpix/3881_Montage_3.jpg 1st row: Full power and 1/2 power 2nd row: 1/4 power and Full power WITH FRESNEL LENS AND FILTER NOT REMOVED I dont have osciloscope to test flash duration. I would say that it matches HBO output (1/1600 sec) ...
by JohnyM
Wed Jan 16, 2019 5:57 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

By the way, if you find useful references such as lists of old cubes/filters/handy fluorophores or equipment documents, I'll put a link in the original post. Send a pm if you prefer. This link that i already posted: https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets ...
by JohnyM
Wed Jan 16, 2019 1:27 am
Forum: Equipment Discussions
Topic: Which camera bodies have fully electronic shutter?
Replies: 39
Views: 11174

It seems to be explained quite well here:
https://www.dpreview.com/articles/58166 ... -to-know/2

Makes sense why Olympus allows flash at very long exposure times.
I guess that with A9II we'll get flash and electronic shutter.
by JohnyM
Tue Jan 15, 2019 8:48 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

I notice the B cube doesn't have at least one filter. That makes sense if they're using the mirror to cut all the short wavelengths. I'll get the cubes together and see what comes out of their colours.. No, that's very bad. No mirror - obviously wont work. No barrier - there will be bleeding. No ex...
by JohnyM
Tue Jan 15, 2019 8:45 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

I do not know what "royal blue" means.
B-1E and B-2E have peak at ~ 470-480nm.
G-2A and G-1A have peak at ~420-430nm.

Both of those cubes would excite chlorophyll.
by JohnyM
Tue Jan 15, 2019 8:38 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

1)On the flash - is that a Nikon fitting? Is the UV filter removed from the flash, or is there just so much light anyway that it's not necessary, or using Wood's glass, or......? 2)Which model 'scope is it - maybe one day? :) 3)I just dismantled an old Sony 3MP camera, only keeping the screws. But ...
by JohnyM
Tue Jan 15, 2019 7:25 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

Cube Excitation Emmision DM580 (BA590) G-2A / G-1A Green Red only DM510 (BA520-560) B-1E / B-2E Blue Green only UV-2A BA420 DM400 UV-2A UV 365nm All the colors B Most common B cube is B-2A You can unscrew excitation filters, you'll be able to read the marking then and can tell for sure which cube yo...
by JohnyM
Tue Jan 15, 2019 7:13 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 10873

This webpage is quite good: https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets Rule of thumb: -Amateur would like autofluorescence (many sparkling colors) so gravitate towards left side of the spectrum and wideband (excitation) longpass (emission) cubes. Multiband cub...