Search found 465 matches

by JohnyM
Sat Feb 09, 2019 10:24 pm
Forum: Equipment Exchange
Topic: WTB Mitutoyo M Plan APO 20X 0.42
Replies: 8
Views: 2689

All i can say from my experience, is that TU Plan Fluors and Plans are excellent corner-corner on APS-C sensor when microscope mounted. Vignettes heavily on anything bigger (which is for sure caused by microscope itself).
Also BD version is one of biggest microscope objective one can get...
by JohnyM
Sat Feb 09, 2019 12:48 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Not enough pixels for 20/0.75?
Replies: 14
Views: 6544

Johny, I see you've edited your last post, And i dont see it, like any other user on this forum. Because i've edited it before it could influence any response. So what exactly is your point behind bringing it up? Mpx count and pixel size are related: you will get the same image with different senso...
by JohnyM
Sat Feb 09, 2019 6:51 am
Forum: Equipment Discussions
Topic: Nikon 50x na .45 VS 50x NA .55
Replies: 6
Views: 2611

Well, then better is TU Plan 50x/0.6 WD 11
Or Mitutoyo 50x/0.55...
Though they all cost way more than old trusty CF's
by JohnyM
Sat Feb 09, 2019 6:44 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Not enough pixels for 20/0.75?
Replies: 14
Views: 6544

You used different "video magnification couplers", hence error in calculations... I did it intentionally to match the FOV to my setups as much as possible, of course it can't be exact. I did it based in the Field Number and adjusting the sensor rectangle to it and I think that it's a good approxima...
by JohnyM
Fri Feb 08, 2019 8:43 pm
Forum: Equipment Discussions
Topic: Nikon 50x na .45 VS 50x NA .55
Replies: 6
Views: 2611

TTL or external light? Aquisition depth?
One is sharper, other have longer WD... Both are better.
by JohnyM
Fri Feb 08, 2019 8:40 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Not enough pixels for 20/0.75?
Replies: 14
Views: 6544

You used different "video magnification couplers", hence error in calculations. For sure, you dont have enough pixels to get all the resolution of 20x/0.75 with 18mpx aps-c.
by JohnyM
Fri Feb 08, 2019 7:07 am
Forum: Equipment Exchange
Topic: Microscope Head - Exchange.
Replies: 8
Views: 2960

Photoport coverage not too big, eh?
by JohnyM
Wed Feb 06, 2019 11:51 pm
Forum: Equipment Exchange
Topic: Photomicroscopic XYZ stage for sale **SOLD**
Replies: 6
Views: 4147

A beauty indeed. Could you write some more about it? Im not gonna buy it, but it looks very impressive and might have some neat tricks we could copy.
by JohnyM
Mon Jan 28, 2019 1:12 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: XY Resolution and Accuracy for Stitching
Replies: 15
Views: 6014

Im 90% done in making XYZ horizontal rig that moves optical train and subject + light remains stationary. Gimme 3 weeks and i'll post details. I ruled out stationary lens method due to lack of optics with big enough image circle. Instead im interested in comparing so called "super resolution" vs pan...
by JohnyM
Mon Jan 28, 2019 7:57 am
Forum: Equipment Discussions
Topic: LED power to the epi-illuminator
Replies: 18
Views: 9897

@ChrisR In microscopes i use - no. Im in a support of good ol' 100W. We do have axio with led epifluorescence and couple of E200 with led. They do have field diaphragm but they also use fly-eye lens to produce uniform led lighting (best solution IMO). I did quite a lot of retrofitting in old constru...
by JohnyM
Mon Jan 28, 2019 6:20 am
Forum: Equipment Discussions
Topic: OK what about Zeiss standard DIC?
Replies: 18
Views: 5421

Check out PZO DIC "UPI". One of most advanced and also cheapest DIC systems i know. Old Zeiss (West) is notorious for delamination. I've bought few pieces of those systems in pristine condition that... still went bad after short (relative) time. On the other hand Zeiss Jena is of excellent quality, ...
by JohnyM
Sun Jan 27, 2019 12:55 pm
Forum: Equipment Discussions
Topic: LED power to the epi-illuminator
Replies: 18
Views: 9897

Well, my illuminators are Zeiss, Nikon, Olympus or Leica engineered. What i think is the problem, is that you place your light source outside of optimal position, without being fully aware of it. So you might have uniform illumination at the cost of power. Overall it's not a big deal, you just produ...
by JohnyM
Sun Jan 27, 2019 10:13 am
Forum: Equipment Discussions
Topic: LED power to the epi-illuminator
Replies: 18
Views: 9897

That's how it's working in all my koehler illuminators.
by JohnyM
Sun Jan 27, 2019 9:57 am
Forum: Equipment Discussions
Topic: LED power to the epi-illuminator
Replies: 18
Views: 9897

That led is already 2x2 and will display ugly cross unless diffused. And when diffused it's power is gonna be roughly equal to 1x light source.
by JohnyM
Sat Jan 26, 2019 6:29 pm
Forum: Equipment Discussions
Topic: Oil immersion condenser
Replies: 25
Views: 7732

Agree. My set is 10x PA, 20xPA, 20xPF, 40x1.3 PF
Exactly because of coverslip correction. Another downside of it is that on stacking you need to constantly readjust it to maintain IQ... and then the focus shifts.