All i can say from my experience, is that TU Plan Fluors and Plans are excellent corner-corner on APS-C sensor when microscope mounted. Vignettes heavily on anything bigger (which is for sure caused by microscope itself).
Also BD version is one of biggest microscope objective one can get...

Johny, I see you've edited your last post, And i dont see it, like any other user on this forum. Because i've edited it before it could influence any response. So what exactly is your point behind bringing it up? Mpx count and pixel size are related: you will get the same image with different senso...

Well, then better is TU Plan 50x/0.6 WD 11
Or Mitutoyo 50x/0.55...
Though they all cost way more than old trusty CF's

You used different "video magnification couplers", hence error in calculations... I did it intentionally to match the FOV to my setups as much as possible, of course it can't be exact. I did it based in the Field Number and adjusting the sensor rectangle to it and I think that it's a good approxima...

TTL or external light? Aquisition depth?
One is sharper, other have longer WD... Both are better.

You used different "video magnification couplers", hence error in calculations. For sure, you dont have enough pixels to get all the resolution of 20x/0.75 with 18mpx aps-c.

Photoport coverage not too big, eh?

A beauty indeed. Could you write some more about it? Im not gonna buy it, but it looks very impressive and might have some neat tricks we could copy.

Im 90% done in making XYZ horizontal rig that moves optical train and subject + light remains stationary. Gimme 3 weeks and i'll post details. I ruled out stationary lens method due to lack of optics with big enough image circle. Instead im interested in comparing so called "super resolution" vs pan...

@ChrisR In microscopes i use - no. Im in a support of good ol' 100W. We do have axio with led epifluorescence and couple of E200 with led. They do have field diaphragm but they also use fly-eye lens to produce uniform led lighting (best solution IMO). I did quite a lot of retrofitting in old constru...

Check out PZO DIC "UPI". One of most advanced and also cheapest DIC systems i know. Old Zeiss (West) is notorious for delamination. I've bought few pieces of those systems in pristine condition that... still went bad after short (relative) time. On the other hand Zeiss Jena is of excellent quality, ...

Well, my illuminators are Zeiss, Nikon, Olympus or Leica engineered. What i think is the problem, is that you place your light source outside of optimal position, without being fully aware of it. So you might have uniform illumination at the cost of power. Overall it's not a big deal, you just produ...

That's how it's working in all my koehler illuminators.

That led is already 2x2 and will display ugly cross unless diffused. And when diffused it's power is gonna be roughly equal to 1x light source.

Agree. My set is 10x PA, 20xPA, 20xPF, 40x1.3 PF
Exactly because of coverslip correction. Another downside of it is that on stacking you need to constantly readjust it to maintain IQ... and then the focus shifts.