Search found 465 matches

by JohnyM
Thu Feb 14, 2019 12:43 pm
Forum: Equipment Discussions
Topic: What is the "Umbra shield" ?
Replies: 4
Views: 2767

Not exactly. It's used on inverted microscope and placed between condenser and subject (assumed petri dish). It's just plain sheet of black material obscuring condenser lens.
by JohnyM
Thu Feb 14, 2019 12:22 pm
Forum: Equipment Discussions
Topic: What is the "Umbra shield" ?
Replies: 4
Views: 2767

Nothing special. Piece of metal. I just use leftover m42 tubes for that purpose.
by JohnyM
Thu Feb 14, 2019 11:35 am
Forum: Equipment Discussions
Topic: Olympus CX41 Objective. Damaged or just bad to begin with?
Replies: 14
Views: 3241

Oh, and BE plans! They are suprizingly good.
by JohnyM
Thu Feb 14, 2019 10:23 am
Forum: Equipment Discussions
Topic: Warning: Don't use Laserpointers on your camera!
Replies: 7
Views: 3255

Re: Warning: Don't use Laserpointers on your camera!

AlxndrBrg wrote:
Antal wrote: BUT:
I got dead pixel since!
Altough I did laser only when mirror were down.
How would the laser create a dead pixel on the sensor if you only ever used it when the mirror was down?
And how would it create a dead pixel on sensor if the mirror was up :lol: ?
by JohnyM
Thu Feb 14, 2019 9:51 am
Forum: Equipment Discussions
Topic: Olympus CX41 Objective. Damaged or just bad to begin with?
Replies: 14
Views: 3241

Zeiss - correction in tube.
Nikon - different size.
Leica - correction in tube (not 100% sure here)

What's left... Olympus :)
by JohnyM
Thu Feb 14, 2019 8:55 am
Forum: Photography Through the Microscope
Topic: Pinus sylvestris autofluorescence stack'n stich
Replies: 9
Views: 1981

Pinus sylvestris autofluorescence stack'n stich

31 fields with 6x on sensor.
Flashgun used to minimize photobleach.

Image
Image
Image
by JohnyM
Thu Feb 14, 2019 8:07 am
Forum: Equipment Discussions
Topic: Warning: Don't use Laserpointers on your camera!
Replies: 7
Views: 3255

Sounds like a coincidence to me.
by JohnyM
Thu Feb 14, 2019 7:09 am
Forum: Equipment Discussions
Topic: Filters for Olympus CX41 (phase, polarization and darkfield)
Replies: 21
Views: 6004

That's correct.
by JohnyM
Thu Feb 14, 2019 6:52 am
Forum: Equipment Discussions
Topic: Filters for Olympus CX41 (phase, polarization and darkfield)
Replies: 21
Views: 6004

While it's fine for polarizer, you'll notice the difference between dedicated quality microscope analyzer and sheet.
by JohnyM
Thu Feb 14, 2019 6:33 am
Forum: Equipment Discussions
Topic: Olympus CX41 Objective. Damaged or just bad to begin with?
Replies: 14
Views: 3241

Oil is special, it's not kitchen or diesel version :wink: More expensive one's are made for fluorescence and high resolution microscopy. Type A should be good for you. While LDF is superior in every aspect, but much more expensive and you wont notice it's traits. Avoid FF type. Probably just search ...
by JohnyM
Thu Feb 14, 2019 5:43 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4100

Subjects respond VERY well to sharpening and "dehaze" feature of CameraRAW. Here are 100% crops from bio parts, that were optimally (usually heavy) processed with those techniques. Reason why bio is problematic with epi TTL is because it's partially reflective and partially transmitting. Why we usua...
by JohnyM
Thu Feb 14, 2019 5:19 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4100

Thanks for input. Thought nobody is interested with it. First comprarision was done with Nikon BD Plan DIC 5x/0.1 through 1,5x teleconverter on APS-C A6500 sensor. Indeed, things look better with higher magnifications, but i have a different theory behind why it's like that. Here's comparision with ...
by JohnyM
Tue Feb 12, 2019 1:08 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Episcopic TTL microscopy compared Bio vs Industrial
Replies: 10
Views: 4100

Episcopic TTL microscopy compared Bio vs Industrial

First of all - images are very soft. Magnifications tend to be higher, while NA's tend to be smaller than bio objectives. But working distances are longer Test subjects are: Bio - semi transparent, semi reflective - Pinus leaf. Industrial - completly opaque - coin. Now results: 1) Darkfield (Top row...
by JohnyM
Sun Feb 10, 2019 10:29 am
Forum: Equipment Discussions
Topic: External shutter controller against photo bleaching
Replies: 8
Views: 4840

So how will you sync light shutter with DSLR shutter? For Zeiss Axio and Nikon HMX lamphouses, it's possible to connect multiple light sources to single collector. Probably Olympus and other brands also offer similar solution. It might be less expensive than attempt to adapt motorized shutter. My pe...
by JohnyM
Sun Feb 10, 2019 7:02 am
Forum: Equipment Discussions
Topic: External shutter controller against photo bleaching
Replies: 8
Views: 4840

What camera you're using and how are you planning to sync it with this shutter?
For me, simplest solution was to implement flash in fluorescence photography. Which is functionally exactly like a shutter, but without all the hassle with electronics.