Not exactly. It's used on inverted microscope and placed between condenser and subject (assumed petri dish). It's just plain sheet of black material obscuring condenser lens.

Nothing special. Piece of metal. I just use leftover m42 tubes for that purpose.

Oh, and BE plans! They are suprizingly good.

AlxndrBrg wrote:

Antal wrote: BUT:
I got dead pixel since!
Altough I did laser only when mirror were down.

How would the laser create a dead pixel on the sensor if you only ever used it when the mirror was down?

And how would it create a dead pixel on sensor if the mirror was up ?

Zeiss - correction in tube.
Nikon - different size.
Leica - correction in tube (not 100% sure here)

What's left... Olympus

31 fields with 6x on sensor.
Flashgun used to minimize photobleach.

Sounds like a coincidence to me.

That's correct.

While it's fine for polarizer, you'll notice the difference between dedicated quality microscope analyzer and sheet.

Oil is special, it's not kitchen or diesel version :wink: More expensive one's are made for fluorescence and high resolution microscopy. Type A should be good for you. While LDF is superior in every aspect, but much more expensive and you wont notice it's traits. Avoid FF type. Probably just search ...

Subjects respond VERY well to sharpening and "dehaze" feature of CameraRAW. Here are 100% crops from bio parts, that were optimally (usually heavy) processed with those techniques. Reason why bio is problematic with epi TTL is because it's partially reflective and partially transmitting. Why we usua...

Thanks for input. Thought nobody is interested with it. First comprarision was done with Nikon BD Plan DIC 5x/0.1 through 1,5x teleconverter on APS-C A6500 sensor. Indeed, things look better with higher magnifications, but i have a different theory behind why it's like that. Here's comparision with ...

First of all - images are very soft. Magnifications tend to be higher, while NA's tend to be smaller than bio objectives. But working distances are longer Test subjects are: Bio - semi transparent, semi reflective - Pinus leaf. Industrial - completly opaque - coin. Now results: 1) Darkfield (Top row...

So how will you sync light shutter with DSLR shutter? For Zeiss Axio and Nikon HMX lamphouses, it's possible to connect multiple light sources to single collector. Probably Olympus and other brands also offer similar solution. It might be less expensive than attempt to adapt motorized shutter. My pe...

What camera you're using and how are you planning to sync it with this shutter?
For me, simplest solution was to implement flash in fluorescence photography. Which is functionally exactly like a shutter, but without all the hassle with electronics.