Now I'm not even sure if my U-PCD is designed for oil immersion or not, it does have a higher NA of 1.25, higher than the U-TLD's 0.9, and the U-PCD-2 at 1.1??? What? So the V2.0 has a lower NA? Can't find any information online, maybe I should try and compare the methods. Surely the oil isn't too ...

Macro_Cosmos wrote: ↑

Fri Aug 14, 2020 6:00 am

What does the "I" even mean here?

I think it's "Iris". As in oil iris objectives.

Hi, - U-DICT: Transmitted DIC module that goes into the nosepiece - U-DICTS: Same as above, but apparently it can be adjusted? However there's no slot for a polariser, and it's not much different to above. Using this would require some extra hardware to hold a polariser, I don't want that mess. Just...

I think it was in a review by Gerald Undone that it was mentioned that shooting stills will contribute to the over-heating issues Hi, Does it say anywhere how the test was done? I couldn't see it in the YouTube review. https://www.youtube.com/watch?v=qDXPiu3wpBs I'm asking because there is another ...

So summarising do you think by using SPlan Apo objectives i lose some image quality or only F.N.? So is it possible to use even higher NA objectives for example 40x on copepods and get a sharp image? I understand that mount preparation has to be perfect, but assuming it is, at what NA does copepods...

For studio use, active cooling might be feasable. There are various setup ideas circulating in the astronomy community for cooling these cameras, e.g. https://www.myastroscience.com/dslrcoolerbox (haven't tried it myself).

Hi Karol, Welcome to the Forum. There are two issues that you need to deal with from the start: Spherical aberration and depth of focus. 1) Spherical aberration https://www.olympus-lifescience.com/de/microscope-resource/primer/java/aberrations/spherical/ prevents you from getting a sharp image of yo...

it must be possible to move a plan apo (before or during stacking) into a lighting dome, due to working distance. Correct? They also write something about this in the paper: "Before constructing the mini-dome, we advise the user to determine the working distance of their lenses for this application...

Based on what paragraph (page number) in the article did you come to your conclusion quote "The light is corrected with the specimen, not the camera+objective. That way the illumination doesn't change as the objective moves during stacking."? Hi Jac, I would say you see this in Fig 5. G+H and in Fi...

FYI, what i mean with a dome lighting setup: A Scalable and Modular Dome Illumination System for Scientific Microphotography on a Budget, See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854399/?fbclid=IwAR1wq8f1aQErUL8CzR3OIaHGYZDUIo-NOXdGThEip6snRYa4julWl7yXK9Q; That's a great paper! It shows wh...

I'm partial to the brass adapters https://www.customphototools.com/macro/ ... s-adapters but unfortunately there is no M52-M25 adapter in stock.

Hi, 1) The idea I described above is the following: The camera sensor is place exactly at the location of the intermediate image in/above the phototube = "direct projection". With the Nikon YT-TV adapter and Nikon 1x C-mount TV adapter A, the intermediate image should be located 17.526 mm above the ...

rjlittlefield wrote: ↑

Wed Jul 29, 2020 11:22 am

Ichthyophthirius wrote: ↑

Wed Jul 29, 2020 1:04 am

does anyone know the NA of the MP-E at 5x?

Wide open, NA 0.149

Thanks, Rik!

NikonUser wrote: ↑

Fri Jul 31, 2020 11:11 am

My objective

Hi,

Which objective was it?

Someone correct me if I'm wrong. It looks to me like all you need to do is to remove the entire camera part and Nikon V-T tube. https://www.lmscope.com/en/Nikon_eclipse_e400_e600_digital_Adapter_en.html Then mount a Nikon YT-TV adapter, a Nikon 1x C-mount TV adapter A and attach a C-mount to M4/3 ad...