Search found 1122 matches

by Ichthyophthirius
Sun Nov 29, 2020 1:24 pm
Forum: Equipment Discussions
Topic: Awakening a Leitz SM microscope
Replies: 22
Views: 2983

Re: Awakening a Leitz SM microscope

To me, the images look great. You clearly have an 'eye' for photography (I also like your photos of the microscopy equipment).

You could move on making your own slides (pond life; diatoms; plant sections etc. are interesting) or experiment with different contrast methods (as mentioned above) or both.
by Ichthyophthirius
Sun Nov 29, 2020 12:02 pm
Forum: Equipment Discussions
Topic: Awakening a Leitz SM microscope
Replies: 22
Views: 2983

Re: Awakening a Leitz SM microscope

Hi Toni, I know that illuminating technics, post-production, quality of sensors are determinant, but I'm afraid that that general optic quality of my tool should be the real limiting factor. I'd like to assure you that this microscope will not be your limiting factor! As long as this microscope and ...
by Ichthyophthirius
Tue Nov 24, 2020 8:13 pm
Forum: Equipment Discussions
Topic: 160mm vs infinity objectives
Replies: 3
Views: 1496

Re: 160mm vs infinity objectives

Hi Henry, For what purpose? Biological objectives for brightfield transmitted illumination? Or more specialised contrast methods? Most major manufacturers switched their biological objectives from 160 mm to infinite tube length in the late 1980s / early 1990s. The early infinite objectives are somet...
by Ichthyophthirius
Fri Oct 30, 2020 10:21 am
Forum: Equipment Discussions
Topic: Carl Zeiss Fluar 10x 0.50 NA lens and tubelens
Replies: 3
Views: 1788

Re: Carl Zeiss Fluar 10x 0.50 NA lens and tubelens

Hi, Zeiss ICS requires a compensating tube lens. https://www.photomacrography.net/forum/viewtopic.php?p=258382 It's explained here: http://www.photomacrography.net/forum/userpix/3229_Zeiss_ICS_1.jpg You will need a tube lens plus the correct tube lens (they vary in diameter because if differences in...
by Ichthyophthirius
Tue Oct 27, 2020 8:46 am
Forum: Equipment Discussions
Topic: Please help me identify this Olympus OEM 60x objective
Replies: 8
Views: 2088

Re: Please help me identify this Olympus OEM 60x objective

Hi, The phase ring is very small for a 60x. So it would probably work with a smaller ring annulus like Ph2 or Ph1 or a custom size. The phase contrast might not we very useful because of the reduced resolution - definitely interesting for experiments. Does it perform as well in brightfield as the se...
by Ichthyophthirius
Sun Oct 25, 2020 4:09 am
Forum: General Discussion Forum and Community Announcements
Topic: Found mantis - now what?
Replies: 4
Views: 1691

Re: Found mantis - now what?

Hi, I found them quite easy to keep. They will feed on almost any insect that doesn't have good defences, so crickets should work. I was feeding mine with foreceps as well so you can even try insects that were previously frozen. You could try attracting and catching some flies outside. She might sti...
by Ichthyophthirius
Sat Oct 24, 2020 6:56 am
Forum: Equipment Discussions
Topic: 10x Objectives: UPlanSApo 10x VS UPlanFL N 10x Ph1
Replies: 8
Views: 2138

Re: 10x Objective with delamination testing

Hi, I agree. This looks as if the black paint, that masks the outer (unused) region of the front lens, has partially rubbed off. I have seen this frequently on 160 mm objectives from Olympus and Nikon. The black masking paint is partially or completely stripped by harsh or too frequent lens cleaning...
by Ichthyophthirius
Fri Oct 23, 2020 12:03 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Nikon 40X 0.55 LWD
Replies: 2
Views: 1593

Re: Nikon 40X 0.55 LWD

Hi, I can't answer your questions directly. But I can say that a priori the BEST way of adapting this lens is to use it without a tube lens of any kind. Just an empty, flocked tube with a length of 150 mm to the sensor will give you the best results. Adding a tube lens will change the working distan...
by Ichthyophthirius
Sun Oct 11, 2020 4:53 am
Forum: Equipment Discussions
Topic: Can I push the resolution of a 1:1 macro lens?
Replies: 23
Views: 4678

Re: Can I push the resolution of a 1:1 macro lens?

Thomas wrote:
Sun Oct 11, 2020 2:33 am
I'm very happy with the RP.
* Stacking works just fine with the Sigma 150mm, as well as
Thanks for the feedback! That's the first confirmation I have found that the focus bracketing feature of the EOS RP works with Sigma lenses. http://www.photomacrography.net/forum/v ... =8&t=41629
by Ichthyophthirius
Sat Oct 10, 2020 5:03 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Flash on the hotshoe
Replies: 5
Views: 2165

Re: Flash on the hotshoe

A large conventional flash on top of the camera can work well if the lens has a long working distance so the flash isn't shaded by the lens barrel. The large distance between flash and subject give you lots of space for installing diffusers. However, it requires a lot of flash power because of the l...
by Ichthyophthirius
Thu Oct 08, 2020 6:12 am
Forum: Equipment Discussions
Topic: Can I push the resolution of a 1:1 macro lens?
Replies: 23
Views: 4678

Re: Can I push the resolution of a 1:1 macro lens?

I use a Canon EOS RP (pixel pitch ~5.76µm) with a Sigma 150mm 1:2.8 APO EX DG HSM macro lens. * Camera (USB tethered to PC) froze repeatedly during stacking. Seems to have problems shifting the lens focus. Hi, I think I will try Lou's suggestion with the teleconverter. But I'm really interested in ...
by Ichthyophthirius
Wed Oct 07, 2020 2:02 pm
Forum: Equipment Discussions
Topic: Sigma 105mm F2.8 DG DN MACRO Art
Replies: 8
Views: 1942

Re: Sigma 105mm F2.8 DG DN MACRO Art

That was a delightful presentation. Thanks for the link!

I can also believe the increase in macro lens sales in Europe during lockdown that he talked about. Certainly confirms my own observations.
by Ichthyophthirius
Fri Oct 02, 2020 11:41 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Eyepiece projection, yes or not??
Replies: 14
Views: 3353

Re: Eyepiece projection, yes or not??

Hi John, You haven't mentioned any microscope yet. I assume you have tried to attach the objective directly to your camera? The Nikon objectives produce an intermediate image of good quality with a diameter of something like 20 ... 25 mm. That's similar to both m4/3 and APS-C sensors. An easy way to...
by Ichthyophthirius
Fri Oct 02, 2020 6:42 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Eyepiece projection, yes or not??
Replies: 14
Views: 3353

Re: Eyepiece projection, yes or not??

Hi John,

Can you also say what camera you use? It helps with telling you exactly what distances to use.
by Ichthyophthirius
Wed Sep 30, 2020 1:35 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Eyepiece projection, yes or not??
Replies: 14
Views: 3353

Re: Eyepiece projection, yes or not??

I agree. The higher the NA, the more important it is to follow in objective labels for tube length and cover glass. Above NA 0.35, presence/absence of the cover glass is important and above NA 0.55, the tube length needs to be within millimeters of the design (for example for direct projection, 150 ...