Search found 5861 matches

by Charles Krebs
Sun May 03, 2020 12:49 am
Forum: Equipment Discussions
Topic: Camera upgrade
Replies: 33
Views: 5262

I've been very happy with the 50D that you suggested years ago too. It's still a very good microscope camera if you don't need video. As I mentioned, but will emphasize again, on a microscope all those pixels (32,5Mp) are really only of use in a quite limited number of cases. Mainly direct projecti...
by Charles Krebs
Sun May 03, 2020 12:04 am
Forum: Equipment Discussions
Topic: Camera upgrade
Replies: 33
Views: 5262

I'm now using a 90D (APS-C) and an EOS-R (full frame). I changed primarily to have a fully electronic shutter in both full frame and APS-C. For continuous light this is fantastic and I would not want to go back to any type of mechanical shutter. But... the reason I asked about flash is that Olympus ...
by Charles Krebs
Sat May 02, 2020 9:03 pm
Forum: Equipment Discussions
Topic: Camera upgrade
Replies: 33
Views: 5262

What lighting do you typically use? Flash, continuous tungsten, continuous LED?

Let me know and I'll have a suggestion or two.
by Charles Krebs
Sat May 02, 2020 12:30 am
Forum: Photography Through the Microscope
Topic: Probable Histiona aroides (video)
Replies: 2
Views: 694

Really good looking 100X phase contrast! Interesting subject.
by Charles Krebs
Sat May 02, 2020 12:28 am
Forum: Photography Through the Microscope
Topic: Ameba Centropyxis (video)
Replies: 2
Views: 754

Good look. You found a nice clean one. So often they are covered in all sorts of debris.
by Charles Krebs
Sat May 02, 2020 12:25 am
Forum: Photography Through the Microscope
Topic: Bottle Brush spores
Replies: 6
Views: 968

Nice, put a print on your wall. :wink:
by Charles Krebs
Sat May 02, 2020 12:23 am
Forum: Photography Through the Microscope
Topic: Diatoms in Circular Oblique Light Update 06/05
Replies: 25
Views: 2985

Fantastic!. The clarity really surprises me for circular oblique. Do I understand correctly... you used a 0.8 NA dry darkfield condenser? (In addition to the lens iris and condenser height).
by Charles Krebs
Sat May 02, 2020 12:13 am
Forum: Photography Through the Microscope
Topic: Freshwater mites
Replies: 14
Views: 1553

These all came out great! First one particularly neat composition.
by Charles Krebs
Sat May 02, 2020 12:09 am
Forum: Photography Through the Microscope
Topic: Mosquito wing details
Replies: 11
Views: 1420

Looks quite good, rendered the fines hairs superbly. This is a great test subject. (What stacking method?) . Working distance is 0.7mm, so yes, this will be something to deal with! (I picked up a BD version awhile ago, but have barely played with yet).
by Charles Krebs
Tue Apr 28, 2020 7:03 pm
Forum: Photography Through the Microscope
Topic: Epi-Fluorescence and Deconvolution
Replies: 7
Views: 1254

Robert,
Really nice shots, and thanks for the article... very informative and helpful.

BTW, if you have not already read them, do a forum search for "deconvolution" and read through 4 posts and threads from RogelioMoreno in 2013. They deal with this topic as well.
by Charles Krebs
Sun Apr 26, 2020 6:35 pm
Forum: Photography Through the Microscope
Topic: Acanthocystis. Desmid.
Replies: 6
Views: 1271

Acanthocystis. Desmid.

From a recent sample. Acanthocyctis. DIC. 40/0.95 S Plan Apo. Canon 90D http://www.krebsmicro.com/forumpix/9734-9744-1024.jpg Acanthocyctis. DIC. 60/1.4 S Plan Apo. Canon 90D http://www.krebsmicro.com/forumpix/cbk_9452sm.jpg Desmid. DIC. 60/1.4 S Plan Apo. Canon 90D http://www.krebsmicro.com/forumpi...
by Charles Krebs
Sun Apr 26, 2020 12:02 am
Forum: Photography Through the Microscope
Topic: Draparnaldia, Fagilaria, and Volvox
Replies: 17
Views: 3695

Thanks all! Saul, The second image (Fragilaria) was from a wet mount where I had scraped off a reddish surface "crud" from some twigs I collected at the edge of a marsh. I searched around the slide and found this arrangement where the diatoms were close to the cover slip. It was a DIC stack using ei...
by Charles Krebs
Sat Apr 25, 2020 11:45 pm
Forum: Photography Through the Microscope
Topic: Asparagus, grape tomato, raspberry and iris seed pod
Replies: 6
Views: 1273

Asparagus, grape tomato, raspberry and iris seed pod

Not my usual subject matter... but kind of fun! Slices. Not super thin, or extremely small. Cut with a vibratome and photographed with a 4X and 10X. This is a section of a "new" Iris flower seed pod. Two 4X frames stitched. Deliberately photographed in a "high key" manner. http://www.krebsmicro.com/...
by Charles Krebs
Sat Apr 25, 2020 11:10 pm
Forum: Photography Through the Microscope
Topic: Fluorescent images of Protists
Replies: 5
Views: 1703

Nice, and an abituous project! The only live "critter" I've illuminated with UV was a Hydra viridis that was on a slide I was working. It fluoresced bright red but completely recoiled at the UV light.
by Charles Krebs
Sat Apr 25, 2020 11:02 pm
Forum: Photography Through the Microscope
Topic: papilio ulysses scales 50x magnification
Replies: 7
Views: 1053

Really excellent image!