Search found 103 matches

by jmc
Fri Nov 20, 2020 4:35 am
Forum: Macro & Microscopy Articles
Topic: Blog on UV and other aspects of microscopy and imaging
Replies: 25
Views: 2222

Re: Blog on UV and other aspects of microscopy and imaging

In case you are interested in a crazy 10x 0.37 UV (365nm) "objective" with a gigantic field size and perfect field flatness, this just came up on eBay today: https://www.ebay.com/itm/193756315611?ul_noapp=true This is quite unique. If nothing else, it is full of UV-transmitting lenses made from exo...
by jmc
Thu Nov 19, 2020 9:31 am
Forum: Macro & Microscopy Articles
Topic: Blog on UV and other aspects of microscopy and imaging
Replies: 25
Views: 2222

Re: Blog on UV and other aspects of microscopy and imaging

My UV microscope build is coming along. The latest post summarises the work to date and brings together links to the different parts to it. Also I've swapped out some of the original Olympus optical components for UV fused silica now, and I share the initial testing of that. Here's the link - https:...
by jmc
Wed Nov 11, 2020 3:19 am
Forum: Equipment Discussions
Topic: Question about window on the underside of Olympus trinocular head
Replies: 25
Views: 3082

Re: Question about window on the underside of Olympus trinocular head

Little bit of an update on this one. As shown above both the prism and the glass window block the short wavelength UV, which means that they will be no good when I am doing UVB imaging. I've taken the plunge and ordered a replacement circular window in UV fused silica. This leaves the issue of the p...
by jmc
Tue Nov 10, 2020 6:10 am
Forum: Photography Through the Microscope
Topic: Gorgonian sea fan spicule in darkfield
Replies: 0
Views: 301

Gorgonian sea fan spicule in darkfield

A Gorgonian sea fan spicule (a prepared Diatoms Labs slide) taken in darkfield. Olympus BHB, 40x A40PL 0.65 lens, darkfield/phase contrast condenser, 2.5x NFK photoeyepiece, 10 images stacked in Zerene. Overall image size reduced for sharing.
2020-11-10-12.54.17 ZS retouched labelled small.jpg
by jmc
Sun Nov 01, 2020 3:21 am
Forum: Equipment Discussions
Topic: Review of Mitakon Creator 85mm f/2.8 1-5X Mark II
Replies: 9
Views: 1117

Re: Review of Mitakon Creator 85mm f/2.8 1-5X Mark II

Nice review Enrico, thanks for sharing.
by jmc
Tue Oct 27, 2020 2:15 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: 1st attempting at modifying a condenser
Replies: 3
Views: 541

Re: 1st attempting at modifying a condenser

Thanks Lou. It's proving to be a lot more involved than I (naively) thought. However I'm gradually working through it.
by jmc
Sat Oct 24, 2020 8:24 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: 1st attempting at modifying a condenser
Replies: 3
Views: 541

1st attempting at modifying a condenser

I'm gradually building a UV microscope for transmission imaging down into the UVB by converting an Olympus BHB. This means swapping out all the original optics for fused silica or similar as glass strongly absorbs the UVB. The condenser has proved a bit of a sticky thing to deal with. I have a Zeiss...
by jmc
Sun Oct 11, 2020 3:13 am
Forum: Equipment Discussions
Topic: DIY Fluorescence with Zeiss fluoro condenser - help needed
Replies: 75
Views: 8654

Re: DIY Fluorescence with Zeiss fluoro condenser - help needed

Viktor, my apologies, I just realised I never responded to this. Yes with 2 layers you should be fine - you'll be down at around 1% transmission. But I'd still recommend wearing UV safety glasses whenever using the light.
by jmc
Fri Oct 02, 2020 7:55 am
Forum: Equipment Discussions
Topic: DIY Fluorescence with Zeiss fluoro condenser - help needed
Replies: 75
Views: 8654

Re: DIY Fluorescence with Zeiss fluoro condenser - help needed

Here's the UV shield I have. Looking at pictures on line I think it's from a Zeiss Axioskop 2 fluorescence microscope - it came with a Zeiss mercury xenon lamp I got. The filter is 3mm thick and plastic. 20201002_151311 small 3mm thick.jpg Some transmission measurements, taken with an Ocean Optics F...
by jmc
Fri Oct 02, 2020 6:39 am
Forum: Equipment Discussions
Topic: DIY Fluorescence with Zeiss fluoro condenser - help needed
Replies: 75
Views: 8654

Re: DIY Fluorescence with Zeiss fluoro condenser - help needed

Would be interesting to see transmission data for commercial UV protection screens, but I haven't found any. I have an orange protection screen, which I believe is a Zeiss one. I'll run it through my spectrometer and see what it looks like. That'd be great! No problem. I've found it. When I've done...
by jmc
Fri Oct 02, 2020 4:37 am
Forum: Equipment Discussions
Topic: DIY Fluorescence with Zeiss fluoro condenser - help needed
Replies: 75
Views: 8654

Re: DIY Fluorescence with Zeiss fluoro condenser - help needed

viktor j nilsson wrote:
Fri Oct 02, 2020 2:51 am
Would be interesting to see transmission data for commercial UV protection screens, but I haven't found any.
I have an orange protection screen, which I believe is a Zeiss one. I'll run it through my spectrometer and see what it looks like.
by jmc
Fri Sep 25, 2020 1:38 am
Forum: Equipment Discussions
Topic: Question about window on the underside of Olympus trinocular head
Replies: 25
Views: 3082

Re: Question about window on the underside of Olympus trinocular head

Scarodactyl wrote:
Thu Sep 24, 2020 11:47 am
jmc wrote:
Thu Sep 24, 2020 8:43 am
Scarodactyl - excuse my ignorance, but what's a 'maler' please?
Phone typo for 'maker'.
Ok, cheers.
by jmc
Thu Sep 24, 2020 8:43 am
Forum: Equipment Discussions
Topic: Question about window on the underside of Olympus trinocular head
Replies: 25
Views: 3082

Re: Question about window on the underside of Olympus trinocular head

Scarodactyl - excuse my ignorance, but what's a 'maler' please? If I put 'maler microscope' into Google nothing really comes up. Pau - yes the plan will be to do UVB transmission microscopy. Absolutely, UVB is incredibly dangerous, and the idea of looking at an unfiltered source containing lots of U...
by jmc
Thu Sep 24, 2020 6:02 am
Forum: Equipment Discussions
Topic: Question about window on the underside of Olympus trinocular head
Replies: 25
Views: 3082

Re: Question about window on the underside of Olympus trinocular head

Unfortunately neither the window nor the beamsplitter are ideal for my UV imaging work - they'll be ok for 365nm work, but certainly no good for UVB imaging. Transmission measured using an Ocean Optics FX spectrometer and deuterium halogen light source. Bino head optics.jpg Both show the characteris...
by jmc
Thu Sep 24, 2020 1:16 am
Forum: Equipment Discussions
Topic: Question about window on the underside of Olympus trinocular head
Replies: 25
Views: 3082

Re: Question about window on the underside of Olympus trinocular head

A few weeks ago I had a microscope arrive with a broken trinocular head (the seller had shipped the microscope with the head still attached and not packed it very well, and trinocular part got pushed into the head). I took the head apart last night. These old Olympuses certainly were built well, but...