Search found 58 matches

by zed
Fri Apr 09, 2021 12:37 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Sunset moth scales @29X
Replies: 11
Views: 511

Re: Sunset moth scales @29X

That is a very brave (and cool) modification!

I have the non-BD version of this lens and it is almost impossible to get diffused reflected light to the sample unless it is perfectly flat. I have mostly given up on this lens in favor of a Nikon 60x/0.7 finite objective because of this.
by zed
Fri Apr 09, 2021 11:20 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Sunset moth scales @29X
Replies: 11
Views: 511

Re: Sunset moth scales @29X

Macrero wrote:
Fri Apr 09, 2021 9:19 am
Also, I have modded the Oly, I sawed (literally) the front part of the barrel in order to get wider illumination angle.
OK - wait - you SAWED your objective? And it still functions?!? Holy cow - do you have a picture of that?
by zed
Fri Apr 09, 2021 7:16 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Sunset moth scales @29X
Replies: 11
Views: 511

Re: Sunset moth scales @29X

Very nicely lit image!

I am always curious about people's lighting setups when using an objective with such a short working distance. Care to share your setup for this image?

Cheers!
by zed
Tue Apr 06, 2021 1:16 pm
Forum: Equipment Discussions
Topic: Mirrorless tethered stacking?
Replies: 7
Views: 478

Re: Mirrorless tethered stacking?

I'm wondering if people have found any great solutions using a Canon or Nikon mirrorless. I've not found anything nearly as simple and reliable as HR (I've tried many tethering apps), but HR only works with Canon and Nikon DSLRs. Great solutions? No. Unfortunately there really is no such software t...
by zed
Mon Mar 29, 2021 5:05 pm
Forum: Equipment Discussions
Topic: Reflected Light - Optiphot Series
Replies: 2
Views: 639

Re: Reflected Light - Optiphot Series

@Scarodactyl - Thanks for the quick response! I had a go at assembling my beamsplitters into a 210mm path and it seems to be working well with the 70/30 I have! It is VERY nice that no additional optics were required. Thanks again!
by zed
Mon Mar 29, 2021 6:45 am
Forum: Equipment Discussions
Topic: Reflected Light - Optiphot Series
Replies: 2
Views: 639

Reflected Light - Optiphot Series

Hi All, I recently started using a Nikon BD MPlan 60X/0.7 finite objective and have found it to be a good addition to my collection. I am interested in adapting a reflected light setup similar to my infinity based system. Rather than reinvent the wheel – I thought I might try to use the lens system ...
by zed
Sun Mar 14, 2021 10:40 am
Forum: Equipment Exchange
Topic: Wanted: Nikon M Plan 60x/0.7 ELWD Objective
Replies: 1
Views: 562

Wanted: Nikon M Plan 60x/0.7 ELWD Objective

Looking to buy one of these objectives - Part#79233

PM if you have one you are willing to part with!

Thank you!
by zed
Sun Feb 14, 2021 9:37 am
Forum: General Discussion Forum and Community Announcements
Topic: Nikon Small World Event - Wistar Institute
Replies: 3
Views: 1079

zed

@zzffnn - Thanks for the compliment and I'm glad you enjoyed the event. I think it is very difficult for imaging scientists to focus energy on the artistic component of this type of work because it is something that is actively discounted within the community. The rise of digital imaging and the rea...
by zed
Wed Feb 10, 2021 8:02 am
Forum: General Discussion Forum and Community Announcements
Topic: Nikon Small World Event - Wistar Institute
Replies: 3
Views: 1079

Nikon Small World Event - Wistar Institute

Hi All, The Wistar Institute in Philadelphia will be hosting a virtual event this Friday 2/12 from 5:00pm-6:00pm EST discussing the art and science of microscopy which I though might be of interest to many on this forum. Nikon Small World Virtual Event: Science Meets Art in the Infinitely Small http...
by zed
Wed Feb 10, 2021 7:54 am
Forum: Equipment Discussions
Topic: Cleanliness of Zeiss 1.5H cover slips
Replies: 2
Views: 841

Re: Cleanliness of Zeiss 1.5H cover slips

Hi Viktor, This is true of all coverslip/slide vendors - even Zeiss. The grease residue from the glass cutters attract all kinds of dust that is impossible to remove prior to packaging. We typically acid wash (1M HCl) with agitation followed by an ethanol rinse to properly prepare our glass for moun...
by zed
Fri Jan 01, 2021 9:02 am
Forum: Equipment Discussions
Topic: Squid Microscope
Replies: 1
Views: 1772

Squid Microscope

Hi All, I thought this pre-print might be of interest to the group here. For those not familiar with Manu's lab - he heads up the Foldscope project ( https://www.foldscope.com/ ) but does a ton of great work making highly functional, low cost microscopy solutions. Squid is a great example of a flexi...
by zed
Thu Oct 15, 2020 5:24 pm
Forum: General Discussion Forum and Community Announcements
Topic: Nikon Small World 2020
Replies: 3
Views: 2440

Re: Nikon Small World 2020

Confocal seems to get the most love. Very few DIC images among the winners. Is the more subtle "DIC look" on the way out in favor of the black&colorful? I wonder if they are getting fewer DIC submissions or if the judges simply prefer the confocal aesthetics? Maybe we will see more DF+Pol images fr...
by zed
Tue Jul 14, 2020 6:20 am
Forum: Equipment Discussions
Topic: AXIOSKOP 2 MOT - SOFTWARE FOR FOCUSING
Replies: 2
Views: 1837

Re: AXIOSKOP 2 MOT - SOFTWARE FOR FOCUSING

Your best bet would be to check out Micro-Manager: https://micro-manager.org/wiki/ZeissCAN Depending on the camera you use there might be some difficulty getting things to work together. I know some have had decent luck with Canon cameras and some older Nikons - but SLR use is not common in the rese...
by zed
Mon Jun 29, 2020 5:27 am
Forum: Equipment Discussions
Topic: Sony ARiV thethering
Replies: 2
Views: 1300

Re: Sony ARiV thethering

Hi Antal, You most certainly can. I use the A7R3 and R4 with USB-C to the computer for control, microUSB to the Stackshot with motorized stage and focus. You can control this best through Zerene (but it does not handle the XY stage). Cognisys has some software to control XY tiling with Z - although ...
by zed
Fri Feb 28, 2020 2:32 pm
Forum: Equipment Discussions
Topic: 300mm tube lens?
Replies: 34
Views: 4418

The Nyquist and 3x criterion apply to monochrome images. Factor in the loss of resolution due to the Bayer array and the number of needed pixels would approximately double. The S1R with 400mm tube lens is not oversampling in this example. Nyquist sampling is 2 times the Rayleigh limit - any more th...