Search found 46 matches

by zed
Tue Jul 14, 2020 6:20 am
Forum: Equipment Discussions
Topic: AXIOSKOP 2 MOT - SOFTWARE FOR FOCUSING
Replies: 2
Views: 429

Re: AXIOSKOP 2 MOT - SOFTWARE FOR FOCUSING

Your best bet would be to check out Micro-Manager: https://micro-manager.org/wiki/ZeissCAN Depending on the camera you use there might be some difficulty getting things to work together. I know some have had decent luck with Canon cameras and some older Nikons - but SLR use is not common in the rese...
by zed
Mon Jun 29, 2020 5:27 am
Forum: Equipment Discussions
Topic: Sony ARiV thethering
Replies: 2
Views: 380

Re: Sony ARiV thethering

Hi Antal, You most certainly can. I use the A7R3 and R4 with USB-C to the computer for control, microUSB to the Stackshot with motorized stage and focus. You can control this best through Zerene (but it does not handle the XY stage). Cognisys has some software to control XY tiling with Z - although ...
by zed
Fri Feb 28, 2020 2:32 pm
Forum: Equipment Discussions
Topic: 300mm tube lens?
Replies: 34
Views: 2696

The Nyquist and 3x criterion apply to monochrome images. Factor in the loss of resolution due to the Bayer array and the number of needed pixels would approximately double. The S1R with 400mm tube lens is not oversampling in this example. Nyquist sampling is 2 times the Rayleigh limit - any more th...
by zed
Tue Feb 25, 2020 12:04 pm
Forum: Equipment Discussions
Topic: 300mm tube lens?
Replies: 34
Views: 2696

OK - You have to give me more than 'RP' :) - what camera is that?

Good luck with everything!
by zed
Tue Feb 25, 2020 10:49 am
Forum: Equipment Discussions
Topic: 300mm tube lens?
Replies: 34
Views: 2696

Your initial claim was that it was not a good idea to use a tube lens longer than 200mm. That's just wrong; if an image is good on APS using a 200mm tube lens, the image is at least as good using a 300mm tube lens on a FF camera with the same or greater number of pixels. Can you agree to that? My i...
by zed
Sun Feb 23, 2020 8:56 pm
Forum: Equipment Discussions
Topic: 300mm tube lens?
Replies: 34
Views: 2696

Let’s assume for a second that you have a 47MP camera (like Lou’s) and a 20x/0.75 objective. For this lens - assuming a white light source: 0.61(500*) / 0.75 = 407nm is the limit of lateral resolution for this optic. * - this value is 1/2 the usable visible spectrum (350nm-650nm) and should increase...
by zed
Sun Feb 23, 2020 12:40 pm
Forum: Equipment Discussions
Topic: 300mm tube lens?
Replies: 34
Views: 2696

While I normally appreciate the experimentation that takes place here on this forum - this is one that doesn’t make sense to me. Mainly because I cannot think of a practical reason why using a tube lens with a focal length longer than what the objective was designed for helps improve image quality. ...
by zed
Wed Feb 19, 2020 7:30 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Exact distance between tube lens and sensor plane?
Replies: 39
Views: 5750

Hi Antonio, From a practical standpoint - I would assemble my tubes so that the Raynox to sensor distance is as close to the 208mm as you can. Then I would attach this to the camera and point it at the moon on a clear night (any phase you can see will do). From there open Live View and adjust the tu...
by zed
Wed Feb 05, 2020 1:12 pm
Forum: Equipment Discussions
Topic: Trying (failing) out a Zeiss Achroplan 40x NA0.75 objective
Replies: 12
Views: 882

These ceramic tip objectives are designed for physiologists who image cells, neurons,etc in fluorescence while simultaneously recording from electrodes patched directly into the cells. This is the reason for the strong taper near the front lens because the objective is exclusively used on upright mi...
by zed
Sun Feb 02, 2020 9:24 am
Forum: Equipment Discussions
Topic: Sony A7R Tethering with Stackshot
Replies: 0
Views: 510

Sony A7R Tethering with Stackshot

Hi All, Anyone out there using a Sony A7R series camera (2-4) with a Stackshot controller AND tethering to a computer? I am considering changing to mirrorless to improve the speed of my acquisitions and it looks like this camera range has only one microUSB port for everything - as opposed to Nikon w...
by zed
Sun Jan 26, 2020 6:24 pm
Forum: Equipment Discussions
Topic: Nikon Modular Focus Block - Regreasing
Replies: 0
Views: 344

Nikon Modular Focus Block - Regreasing

Hi All, I recently bought a Nikon modular focus block from Robert O'Toole (Labophot 1 era) and have successfully direct coupled a motor along with upgrading to lothman's metal pinion gear. The device is working fine now - however the course drive is very sticky and I'm considering tearing it down co...
by zed
Sat Jan 25, 2020 8:16 am
Forum: Equipment Discussions
Topic: NA 0.9 vs NA 1.4 DIC is it worth to go for it?
Replies: 5
Views: 528

The answer to your question depends on what you are imaging and the resolution that is required for the subject matter (which you don't mention). All else being equal a 1.4 oil optic will give you a significant resolution bump but also significant contrast improvement - if done correctly. Higher NA ...
by zed
Wed Jan 15, 2020 8:32 pm
Forum: Equipment Discussions
Topic: Best tube choices to accompany Mitutoyo 5x and 10x
Replies: 13
Views: 1804

Just my 2 cents - the Raynox DCR-150 works fantastically with a range of Mitutoyo optics. I use the 5x, 10x and 20x with the DCR-150 as a tube lens and have yet to find a better performer. I tested against the Thor TTL-200 and was unimpressed with this optic as a tube lens on a full frame camera (I ...
by zed
Mon Jan 06, 2020 4:45 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Phanaeus triangularis - Dung Beetle
Replies: 2
Views: 905

Phanaeus triangularis - Dung Beetle

https://photos.smugmug.com/Microscopy/i-K8hRxNj/0/f7c47ef6/XL/DungBeetle_1500-XL.jpg Found this little guy in the pool filter a few months ago. It dried out with some cool patterns in the eye that add a bit of drama. The color in the eye was tweaked a bit in PS. 5x Mitutoyo with Nikon D850. 225 ima...
by zed
Sat Dec 21, 2019 10:18 pm
Forum: Equipment Discussions
Topic: Zeiss C-Apochromat 40x 1.20W Sharp Centre
Replies: 6
Views: 750

Hi Ichty, If you are using a Zeiss microscope along with an SLR or mirrorless camera of some sort (does not matter) - you are likely already using a photo eyepiece or some other optic that reduces the field of view - so the comment that the ‘Plan-Apochromat series are absolutely crisp all the way to...