Search found 92 matches
- Fri Mar 29, 2024 1:03 pm
- Forum: Equipment Discussions
- Topic: BX41 focus assembly
- Replies: 4
- Views: 266
Re: BX41 focus assembly
AFAIR you should be able to remove the FF assembly by holding one FF knob and unscrewing the other. Then you can pull out the fine focus axle from the drive shaft (through hole in the coarse focus) It is coupled to the coarse focus via a planetary gear in the (I think) right hand knob. The screw hol...
- Fri Mar 08, 2024 2:51 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Batch processing multiple stacks located in the same folder using Zerene Stacker
- Replies: 4
- Views: 2994
Re: Batch processing multiple stacks located in the same folder using Zerene Stacker
So, I am now considering what a good practical facility should look like, if I were to add one into Zerene Stacker. I would be interested to hear more from you and other people about what data organizations you have and what you'd like to do with them. I really would love to see a batch facility in...
- Mon Jan 16, 2023 7:33 am
- Forum: Beginners Micro
- Topic: New guy here with Olympus question
- Replies: 11
- Views: 3835
Re: New guy here with Olympus question
Regarding ergonomics: The biggest thing for me in the BX world were the extra thick rubber sleeves for the coaxial table drive.
In recent times there was an add-on to extend the standard height (BX43/53 and surely BX40) table controls down to desk height. This is also very comfortable.
In recent times there was an add-on to extend the standard height (BX43/53 and surely BX40) table controls down to desk height. This is also very comfortable.
- Mon Jan 16, 2023 7:30 am
- Forum: Beginners Micro
- Topic: New guy here with Olympus question
- Replies: 11
- Views: 3835
Re: New guy here with Olympus question
Hi, i cant really follow why the BX40 stand is regarded as so much inferior. The light source is lower power (as is the source in the BX41 and 43) and the objective revolver is not interchangable in the default configuration. Also in contrast to the BX50 it does not have filters in the transmitted i...
- Tue Dec 20, 2022 12:28 pm
- Forum: Equipment Discussions
- Topic: (Help) DIY motorized microscope stage
- Replies: 15
- Views: 2985
Re: (Help) DIY motorized microscope stage
Hi,
i cannot give you first hand experience (i use a motorized prior stage) but these might give you some pointers:
https://github.com/openUC2/UC2-Motorized-XY-Table
https://theo.io/blog/2018/02/01/save-99 ... ge-for-60/
Peter
i cannot give you first hand experience (i use a motorized prior stage) but these might give you some pointers:
https://github.com/openUC2/UC2-Motorized-XY-Table
https://theo.io/blog/2018/02/01/save-99 ... ge-for-60/
Peter
- Thu Dec 15, 2022 2:44 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Amateur uses for fluorescence microscopy?
- Replies: 10
- Views: 2002
Re: Amateur uses for fluorescence microscopy?
You can get pretty far with micro-manager (https://micro-manager.org/) its a free and open source control software for microscopes (everything from hobby usb cameras to fancy custom build setups). chances are good that you can get the fw camera working. it has a broad range of drivers (also industri...
- Thu Dec 15, 2022 1:56 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Amateur uses for fluorescence microscopy?
- Replies: 10
- Views: 2002
Re: Amateur uses for fluorescence microscopy?
Hi, this looks like a standard setup for in situ hybridisation (e.g. for genetics or pathology). DAPI is used as a counterstain in many fixed pathological/biological samples. Spectrum green and spectrum orange are proprietary fluorophores used by abbott. These filter sets are very "narrow" and give ...
- Sat Sep 17, 2022 11:52 am
- Forum: Photography Through the Microscope
- Topic: Software question
- Replies: 5
- Views: 1135
Re: Software question
You need to know how many pixels correspond to a micrometer or vise versa. Best get an objective micrometer, these used to be expensive but now come quite cheaply from china and are adequately for nearly all purposes. Take a picture of the objective micrometer (or any other feature with a known size...
- Fri Sep 16, 2022 6:07 am
- Forum: Photography Through the Microscope
- Topic: Software question
- Replies: 5
- Views: 1135
Re: Software question
This might better fit in the technical forum
You need some sort of pixel calibration (know how many µm is your pixel). ImageJ can make this easy for you (https://kaplinskylab.domains.swarthmore ... alebar.htm). If you want to have more aesthetic control you have to do it manually.
You need some sort of pixel calibration (know how many µm is your pixel). ImageJ can make this easy for you (https://kaplinskylab.domains.swarthmore ... alebar.htm). If you want to have more aesthetic control you have to do it manually.
- Wed Aug 10, 2022 5:23 am
- Forum: Photography Through the Microscope
- Topic: Fibroblast cells with and without deconvolution
- Replies: 13
- Views: 1580
Re: Fibroblast cells with and without deconvolution
If someone wants to try deconvolution (and has matlab) at home there is a quite recent publication by Guo et al. "Rapid image deconvolution and multiview fusion for optical microscopy" https://www.nature.com/articles/s41587-020-0560-x which also offers ready to use code. Compared to the previously a...
- Sun Mar 07, 2021 2:07 am
- Forum: Equipment Discussions
- Topic: Olympus U TLU tube lens vs SWTR-2 Superwide Head
- Replies: 11
- Views: 2771
Re: Olympus U TLU tube lens vs SWTR-2 Superwide Head
Thank you for this test. It is really helpful (i have been one of the guys suggesting that the superwide heads might deliver better quality at the edges of the image but never had the time to formally test it). Officially the TLU is for 22 mm but it odes not surprise me that it works beyond that. ma...
- Wed Feb 10, 2021 2:38 am
- Forum: Equipment Discussions
- Topic: Anyone had any experience with LM Microscope camera adapters?
- Replies: 3
- Views: 1293
Re: Anyone had any experience with LM Microscope camera adapters?
I have no first hand experience but LM Scope was the company our Olympus technician recommended as first pick for DSLR adaption. Also said that they provide excellent support and reccomendations.
They are pricey though.
They are pricey though.
- Fri Feb 05, 2021 6:32 am
- Forum: Equipment Discussions
- Topic: Even DF illumination for 4x objective
- Replies: 17
- Views: 4195
Re: Even DF illumination for 4x objective
If you have Phase contrast a PH3 or PH2 ring should also provide DF on 4x.
- Sat Jan 16, 2021 12:47 pm
- Forum: Equipment Discussions
- Topic: fluorescence metoo
- Replies: 9
- Views: 4018
Re: fluorescence metoo
I cannot agree more with jmc and others. you should be careful with what you do (and follow the instructions in the manual closely). There are some specifics like "not touching the glass" and not looking into the unshielded lightsource that come with using a arc source. Osram ans Ushio both make bul...
- Thu Dec 24, 2020 2:38 am
- Forum: Photography Through the Microscope
- Topic: Onion
- Replies: 23
- Views: 9357
Re: Onion
Spectacular images. Thank you for sharing.