Search found 88 matches

by billjanes1
Fri Jul 17, 2020 7:51 am
Forum: Beginners Micro
Topic: staining blood with giemsa
Replies: 19
Views: 1752

Re: staining blood with giemsa

Sorry to hear that you have CLL. Fixation preserves and stabilizes the specimen prior to processing. For blood and bone marrow smears absolute methanol is generally used. For tissue biopsies formaldehyde is often used. For giemsa staining the smear is fixed in methanol and then placed in diluted gie...
by billjanes1
Thu Jul 16, 2020 8:53 am
Forum: Beginners Micro
Topic: staining blood with giemsa
Replies: 19
Views: 1752

Re: staining blood with giemsa

I am now retired but have had extensive experience in the hematology lab as a pathologist. Your blood smear is very poor. Too thick with extensive Rouleaux formation (stack of coins). To make a good smear you put a small drop of blood near one end of the slide and then use another slide to make the ...
by billjanes1
Tue Jun 04, 2019 3:30 pm
Forum: Equipment Discussions
Topic: Need advice about tube lens for infinite objectives
Replies: 20
Views: 4326

Re: Wemacro Tube Assembly with Cognisys Quick Release Clamp

I’ve read about the raynox 150 as a tube Raynox 150 makes an excellent tube lens. See http://www.photomacrography.net/forum/viewtopic.php?t=35350 for some discussion about how to make a tube assembly out I see that WeMacro now sells a Raynox tube lens assembly, https://www.wemacro.com/?product=rayn...
by billjanes1
Fri May 17, 2019 4:47 am
Forum: Equipment Discussions
Topic: DSLRs with full electronic shutter?
Replies: 13
Views: 2623

Re: DSLRs with full electronic shutter?

I am nearing the rated shutter life of my Canon 6D, and am starting to think about a replacement. Are there any DSLRs other than the Pentax K1 that have a full electronic shutter mode? It seems wasteful to me to use a mechanical shutter for macro work when most DSLRs are rated for <200,000 shots. A...
by billjanes1
Fri Mar 29, 2019 8:54 am
Forum: Photography Through the Microscope
Topic: Dog Urinary Bladder
Replies: 3
Views: 1185

That looks more like an image if the gall bladder, not urinary bladder. The image shows columnar epithelium with a papillary pattern. The urinary bladder has transitional epithelium. Here is an image of human gall bladder from the Iowa Histopathology collection for comparison. http://www.photomacrog...
by billjanes1
Fri Jan 04, 2019 9:04 am
Forum: Equipment Discussions
Topic: Canon MPE 65 + Raynox MSN 202 or MPE plus Extension tubes
Replies: 10
Views: 3768

Re: Canon MPE 65 + Raynox MSN 202 or MPE plus Extension tube

Hello, For stacking purposes I am using the MPE till the 5x range. Beyond 5x I have recently started experimenting usage of MSN 202 over and above the MPE. As I understand raynox having its own optics, things look visibly hazy/blurry as when we see the MSN attached to a regular lens/macro lens. I h...
by billjanes1
Sat Dec 29, 2018 7:31 am
Forum: Equipment Discussions
Topic: Mitutoyo m planapo 10x with m25 thread?
Replies: 5
Views: 1766

Lou Jost wrote:
The Mitty objective has RMS threads
That's wrong, at least for any M Plan Apo Mitu objective I've ever seen.
I stand corrected. The Mitutoyo objective threads are M26 x 36 TPI (threads per inch). This pitch is the same as RMS, but the diameter is different.

Bill
by billjanes1
Sat Dec 29, 2018 6:15 am
Forum: Equipment Discussions
Topic: Mitutoyo m planapo 10x with m25 thread?
Replies: 5
Views: 1766

Re: Mitutoyo m planapo 10x with m25 thread?

I recently scored a mitty 10x on eBay for a good price. I already had an m25 objective adapter for my z16, and an m25 to m26 adapter, which allowed me to hook up a Hirox objective to it. Howevwr, when I got the mitutoyo in today I was surprised to find its threads were too small for the m26 adapter...
by billjanes1
Fri Jun 22, 2018 8:50 am
Forum: Equipment Discussions
Topic: Nikon Plastic Case.. where I can buy???
Replies: 3
Views: 1548

Re: Nikon Plastic Case.. where I can buy???

Does anyone know where I to buy original plastic boxes Nikon CFI 60 .... ??? only plastic box - ORYGINAL. Thread M25 or RMS. ??? http://www.photomacrography.net/forum/userpix/6734_20180611_140344_1.jpg Amazon (USA) has cases that fit RMS objectives at a reasonable price, but I do not know if they c...
by billjanes1
Wed May 02, 2018 9:07 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Failing Zerene 101
Replies: 6
Views: 2548

It doesn't depend which stacking software you use as such, it depends how you expose and align your images. The way to avoid it is to make sure the whole field of view you care about is included at both ends of the stack, when you set it up. As I understand things, when focusing by ring it is best ...
by billjanes1
Sun Feb 25, 2018 5:12 pm
Forum: Equipment Discussions
Topic: EFCS & Flash in LV Circuit
Replies: 29
Views: 7248

Bill, Many times I find the Japanese & Chinese manuals difficult to interpret (Yonguno is one of the worst), and Nikon is certainly not the best I've seen as with my prior cameras. So I generally do trial and error testing to find out how stuff works after reading the manual multiple times, then go...
by billjanes1
Sun Feb 25, 2018 5:03 pm
Forum: Equipment Discussions
Topic: EFCS & Flash in LV Circuit
Replies: 29
Views: 7248

Think I know why Nikon doesn't allow flash while in EFCS mode in Live View, or at least a reason to be cautious when using such. Since EFCS has no conventional shutters... I'm confused. My understanding is that EFCS = Electronic First Curtain Shutter, with the second curtain being mechanical as alw...
by billjanes1
Sun Feb 25, 2018 5:42 am
Forum: Equipment Discussions
Topic: EFCS & Flash in LV Circuit
Replies: 29
Views: 7248

Think I know why Nikon doesn't allow flash while in EFCS mode in Live View, or at least a reason to be cautious when using such. Since EFCS has no conventional shutters... I'm confused. My understanding is that EFCS = Electronic First Curtain Shutter, with the second curtain being mechanical as alw...
by billjanes1
Thu Feb 15, 2018 11:25 am
Forum: Equipment Discussions
Topic: Longitudinal Chromatic Aberration 1X Macro Lens Test
Replies: 42
Views: 7405

Re: Longitudinal Chromatic Aberration 1X Macro Lens Test

Please refer to Rik's table below for some details. Providing some additional links... The referenced page is at http://zerenesystems.com/cms/stacker/docs/troubleshooting/ringversusrail . The blue-background slide appears to be an extract from one of my PowerPoint presentations, perhaps slide 24 of...
by billjanes1
Thu Feb 15, 2018 9:37 am
Forum: Equipment Discussions
Topic: Longitudinal Chromatic Aberration 1X Macro Lens Test
Replies: 42
Views: 7405

Re: Longitudinal Chromatic Aberration 1X Macro Lens Test

What makes the Voigtlander 125 such a great macro lens is the fact it is unusually-sharp wide-open and razor-sharp by f/4 (just look at it compared to the Sigma). When you add to this exceptional bokeh and color-rendering qualities, along with something like 600-degrees of focus throw, you have a l...