Search found 17983 matches
- Mon Jan 18, 2021 12:43 pm
- Forum: Photography Through the Microscope
- Topic: ...two little babies on the way...
- Replies: 3
- Views: 65
Re: ...two little babies on the way...
Thanks for including the original. That matches pretty close to what I got by inverting the lightness channel in an Lab version of your post at top of thread. But it does not match exactly, so now I am curious. I notice that in your original, the eyes are very dark, almost black. With inverted lumin...
- Mon Jan 18, 2021 10:41 am
- Forum: Photography Through the Microscope
- Topic: ...two little babies on the way...
- Replies: 3
- Views: 65
Re: ...two little babies on the way...
Excellent -- and that is a very clever trick, to invert just the luminosity channel so as to convert brightfield to darkfield while preserving colors!
--Rik
--Rik
- Sun Jan 17, 2021 10:45 am
- Forum: Equipment Discussions
- Topic: 50X objective recommendation
- Replies: 29
- Views: 902
Re: 50X objective recommendation
With regards to focus blocks, the well regarded Olympus BHMJ is actually quite expensive. Would something much cheaper (e.g. https://www.ebay.com/itm/Stereo-Microscope-Adjustment-Coaxial-Coarse-and-Fine-Focusing-Arm-E-Head-Holder/273448954661) be equally suitable/capable? Very interesting! Today's ...
- Thu Jan 14, 2021 12:12 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Looking for advice to improve sharpness
- Replies: 7
- Views: 268
Re: Looking for advice to improve sharpness
Viewing your image at full resolution (5496 x 3864), I see a lot of lateral chromatic aberration -- color fringes away from center on light vs dark transitions. That is a property of the objective. You can eliminate the obvious fringing with post-processing in Photoshop and similar tools, but fringi...
- Sun Jan 10, 2021 9:31 pm
- Forum: General Discussion Forum and Community Announcements
- Topic: Having trouble uploading avatar
- Replies: 4
- Views: 163
Re: Having trouble uploading avatar
In CS4, I believe it was File > "Save for Web & Devices", so directly under the File menu, not under Export.
--Rik
--Rik
- Sun Jan 10, 2021 6:50 pm
- Forum: General Discussion Forum and Community Announcements
- Topic: Having trouble uploading avatar
- Replies: 4
- Views: 163
Re: Having trouble uploading avatar
What's making your files too large is metadata embedded in them. Get rid of that and there should be no trouble with size. In Photoshop, the simplest approach is to save the image using File > Export > Save for Web (Legacy). Using GIMP, follow the instructions at https://www.makeuseof.com/tag/3-ways...
- Sun Jan 10, 2021 11:38 am
- Forum: Photography Through the Microscope
- Topic: Sea Urchin Spines SEM
- Replies: 7
- Views: 347
Re: Sea Urchin Spines SEM
Thanks for the detail about preparation. About the colors, I have seen Pau's approach used a few times. Doing it fully automatically is indeed tricky because you have to match perspective -- not just viewing angle but also distance from center of perspective to the subject. This requires a careful m...
- Sat Jan 09, 2021 10:27 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Help with Zerene Stacker
- Replies: 11
- Views: 426
Re: Help with Zerene Stacker
Thank you for the additional information. These images look great. I like to hear your thoughts on the fuzziness around the hairy portion of the seed. Do you think if that can still be minimized further? If so, how? I assume that by "fuzziness" you refer to areas like I have marked here, where it se...
- Sat Jan 09, 2021 8:27 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Help with Zerene Stacker
- Replies: 11
- Views: 426
Re: Help with Zerene Stacker
Great!
Can we see the final image, please? I am very curious what this subject looks like (and what it is, for that matter).
Also it would be great to get some information about magnification, step size, number of frames, and so on.
--Rik
Can we see the final image, please? I am very curious what this subject looks like (and what it is, for that matter).
Also it would be great to get some information about magnification, step size, number of frames, and so on.
--Rik
- Sat Jan 09, 2021 12:53 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Help with Zerene Stacker
- Replies: 11
- Views: 426
Re: Help with Zerene Stacker
I notice three things that seem relevant: 1. In the right-hand image, the front structure is very high contrast and is surrounded by a discolored edge. This suggests an extreme value of "brightness" adjustment, caused by having some source frames that are very different from other source frames. 2. ...
- Sat Jan 09, 2021 11:51 am
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: mountain swallowtail
- Replies: 9
- Views: 410
Re: mountain swallowtail
I have compared DMap interpolated with "Bicubic", "Lanczos2", "Lanczos4" and found no difference. But in general, DMap performance is much better than PMax, regardless of interpolation method. Does it depend on the blue scales or my setup? In general, DMap is better at handling simple geometries th...
- Fri Jan 08, 2021 5:55 pm
- Forum: Equipment Discussions
- Topic: Raynox dcr150 custom tube lens
- Replies: 21
- Views: 725
Re: Raynox dcr150 custom tube lens
I am curious about the motivation for custom design & build.
Why not just assemble a flocked tube from standard parts, for example as in viewtopic.php?t=35350 ?
--Rik
Why not just assemble a flocked tube from standard parts, for example as in viewtopic.php?t=35350 ?
--Rik
- Fri Jan 08, 2021 5:34 pm
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: mountain swallowtail
- Replies: 9
- Views: 410
Re: mountain swallowtail
best method (Lanczos3, Lanczos8, etc.) to interpolate the blue scales? At 50X NA 0.55, you're running at effective aperture f/45 (=50/(2*0.55)). Even for extreme blue wavelength 400 nm, that still puts the diffraction cutoff frequency at 18.2 microns per cycle. Against that, your camera has a senso...
- Thu Jan 07, 2021 3:51 pm
- Forum: Photography Through the Microscope
- Topic: Sea Urchin Spines SEM
- Replies: 7
- Views: 347
Re: Sea Urchin Spines SEM
Very nice!
I'm curious, what sort of preparation did these have to go through, in order to be imaged?
--Rik
I'm curious, what sort of preparation did these have to go through, in order to be imaged?
--Rik
- Mon Jan 04, 2021 8:18 pm
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: Windows of Papilio ulysses wing scale
- Replies: 16
- Views: 1765
Re: Windows of Papilio ulysses wing scale
I notice that one of the earlier links is now dead (https://core.ac.uk/download/pdf/12825066.pdf). But I found what I think is the same reference, via https://www.researchgate.net/publication/5495569_Sculpted-multilayer_optical_effects_in_two_species_of_Papilio_butterfly . The RIS citation and abstr...