Search found 19 matches

by Luis Barrera Puigdollers
Thu Feb 25, 2021 8:03 am
Forum: Photography Through the Microscope
Topic: First attempt at a thin section with a manual microtome
Replies: 10
Views: 2542

Re: First attempt at a thin section with a manual microtome

The bench microtome you mention should work very well. I never use a handheld microtome holding it with my left hand, so the cutting precision is not obtained in my opinion and it needs a clamping to a workbench, table or kitchen counter.
by Luis Barrera Puigdollers
Wed Feb 24, 2021 9:47 am
Forum: Photography Through the Microscope
Topic: First attempt at a thin section with a manual microtome
Replies: 10
Views: 2542

Re: First attempt at a thin section with a manual microtome

Hi, Before buying a rotary microtome you should consider several things: the purpose of course (animal or plant tissue), what type of blade will you use ( the large blade with triangular shape difficult to sharpen and expensive or the thin disposable and cheap) what type of paraffin block (cube-shap...
by Luis Barrera Puigdollers
Tue Feb 23, 2021 11:15 am
Forum: Photography Through the Microscope
Topic: First attempt at a thin section with a manual microtome
Replies: 10
Views: 2542

Re: First attempt at a thin section with a manual microtome

Hi again,
With a manual microtome, the blade and "cutting art" are critical. I never use the blade standard of a microtome. I use a razor blade mounted on a thick handle like glass scrappers to best control
by Luis Barrera Puigdollers
Tue Feb 23, 2021 11:02 am
Forum: Photography Through the Microscope
Topic: First attempt at a thin section with a manual microtome
Replies: 10
Views: 2542

Re: First attempt at a thin section with a manual microtome

Hi, In my humble opinion, a very thin section is useful for botanists but lacks the beautiful 3D look for a considered photogenic image. I've tried with embedded paraffin and a lab microtome ,and sections over 20 microns usually roll up on themselves making them useless, but is not impossible. Not a...
by Luis Barrera Puigdollers
Mon Feb 01, 2021 12:30 pm
Forum: Photography Through the Microscope
Topic: Abalone shell
Replies: 5
Views: 2477

Re: Abalone shell

Amazing! I always like your artistic way of presenting your photos whatever the subject of attention. This makes the difference, the sense of art, much more than a technique
by Luis Barrera Puigdollers
Fri Oct 09, 2020 5:25 am
Forum: Photography Through the Microscope
Topic: Algal bloom under the microscope
Replies: 11
Views: 3979

Re: Algal bloom under the microscope

Amazing and super photogenic subject!!!I envy your equipment . I hope people watching this could control GAS
by Luis Barrera Puigdollers
Fri Oct 09, 2020 5:12 am
Forum: Photography Through the Microscope
Topic: Amoeba proteus
Replies: 6
Views: 3423

Re: Amoeba proteus

Well done!! Thanks for sharing
by Luis Barrera Puigdollers
Wed Sep 30, 2020 2:07 am
Forum: Photography Through the Microscope
Topic: Diatoms: Star and Triangle shaped
Replies: 7
Views: 2362

Re: Diatoms: Star and Triangle shaped

Wow, excellent images for me!!! Could you tell us more about your setup? Direct projection means that you don´t use the trinocular head, nor relay lens and the camera is directly attached over the nosepiece, right? So, the 100x magnification belongs entirely to your objective. The same magnification...
by Luis Barrera Puigdollers
Tue Sep 08, 2020 11:48 am
Forum: Photography Through the Microscope
Topic: Hibiscus rosa-sinensis pollen
Replies: 12
Views: 3030

Re: Hibiscus rosa-sinensis pollen

Thanks Bob. Some days ago I was thinking about SEM for pollen study. Well I think I would gain in detail, resolution and 3D vision , but the beauty and color of those almost crystalline spicules would be lost
by Luis Barrera Puigdollers
Wed Sep 02, 2020 2:12 pm
Forum: Photography Through the Microscope
Topic: Hibiscus rosa-sinensis pollen
Replies: 12
Views: 3030

Re: Hibiscus rosa-sinensis pollen

Many thanks Ken and Luis
by Luis Barrera Puigdollers
Sat Aug 29, 2020 12:27 pm
Forum: Photography Through the Microscope
Topic: Diatom Ace "Prize Medal": Coconeis
Replies: 3
Views: 1558

Re: Diatom Ace "Prize Medal": Coconeis

Looks great !! A.C. Cole would be happy to see your work , but probably never could see it like that and less at 100x
by Luis Barrera Puigdollers
Sat Aug 29, 2020 12:19 pm
Forum: Photography Through the Microscope
Topic: Inclusions in Baltic Amber: Midge ( ♂️)
Replies: 5
Views: 1924

Re: Inclusions in Baltic Amber: Midge ( ♂️)

Something special, a window to the past. Thanks for sharing
by Luis Barrera Puigdollers
Sat Aug 29, 2020 1:35 am
Forum: Photography Through the Microscope
Topic: Hibiscus rosa-sinensis pollen
Replies: 12
Views: 3030

Re: Hibiscus rosa-sinensis pollen

Hi Rik Yes, it was a mistake. Because I knew the process I assume my mind directed my fingers. It is a whole Dmap from top view to bottom and some partials Dmap of 50 pics all stacked with Pmax. After that retouched layer by layer to retrieve information or improve it. Nothing special I think, I'm s...
by Luis Barrera Puigdollers
Fri Aug 28, 2020 1:38 pm
Forum: Photography Through the Microscope
Topic: Hibiscus rosa-sinensis pollen
Replies: 12
Views: 3030

Re: Hibiscus rosa-sinensis pollen

Welcome, that's a really nice pollen, looks like a certain human malware that's still roaming. :shock: Excellent details! Looks like a piece of crystal. (Minor correction: It's Dmap, not Dmax) Thanks for your appreciation. In fact, it is a personal Dmap/Pmax to prevent noise accumulation ,overlappi...