Have you ever tried different excitation wavelengths with your setup? Do you have to refocus the grating for this? Peter I haven't tried different wavelenghs, but that wouldn't be a problem. Any inchoerent light source is very suitable for structured illumination. Refocusing the grating is very eas...

Thanks!

You can find many in the gallery and in the articles!

Hello! I've just released my personal blog about optical and electron microscopy. I decided to share my experiences with my instruments, so maybe they will be of help for people who like to experiment new horizons in microscopy, modify their insturments and build new ones. You will find topics like ...

Amazing shots!

How did you prepare the samples? Are them under a coverslip or dry?
What are the specs of your 6,3x objective?

Wow I wish I knew how to find those beuatiful marine forms!

Thanks for explaining!

Very good job!

Thanks for your answer Jacek! Those are excellent objectives. I also have one Nikon planapo CFN 20x and it's amazing! I mean the "pads" you are talking about here: "Water mites is a preparation on the pads, so they do not crush, I put two pads of thick cover slips. On top put a thin glass 0.17 mm. "...

Very beautiful shots.

Could you give some more information about your setup?

Could you post a picture of your microscope?

What's the brand/specs of your 6,3x objective?

What are the "pads" you are using to prepare the specimens?

Hello! I've been practicing this technique in the last months and so far these are some of the slides I menaged to make. Unfortunatly my low magnification setup is not good and pictures' quality is low, but they can still give an idea of the composition! http://www.photomacrography.net/forum/userpix...

Very nice!
Do you have any tips where to find those amoebas or would you consider sending a sample to Italy?

Very interesting!
What kind of excitation/barrier filters did you use for fluorescence shots?

Hello!
I'm looking for DIC sliders that can match my Zeiss inko condenser (the two prisms version).
http://i.ebayimg.com/00/s/MTAxN1gxNjAw/ ... U/$_57.JPG

Any help would be really appreciated!