Well I would like to use some old manual Nikon lenses (not for macro), definitely a 400mm and possibly 300mm and 200mm too. Those (particularly the old 400mm f/5.6 ED-IF) make good tube lenses when you need more magnification. Of course resolution on the subject doesn't increase when using using su...

I have a different philosophy than Macro_Cosmos. The wonderful thing about mirrrorless cameras is the freedom to use any brand of lens. Lens mounts (even good ones) are inexpensive compared to lenses, so I have a wide varierty of mounts. I can use any of my old legacy Nikon lenses, plus old manual F...

Don't forget that you can use a slightly longer lens as the tube lens. What is the sensor size of your old camera? If it is an APS sensor, remember that a 300mm tube lens on FF will give you the same FOV as a 200mm on APS.
Lou

Chris, that's an interesting observation. Infrared hot spots behave this way: they get worse on stopping down, and are worst in the center. I wonder if something similar is happening here. One partial solution in IR photography is the use of a very exact lens hood, so no stray light gets in. Forum m...

Good question

Actinoptychus heliopelta from Dunkirk, Maryland, USA, for example can be rather large.

Do you know if that would be a terrestrial one or an aquatic one?

It sure looks good!

I have most of them also without the backlight. I shot double stacks. ;-)

Excellent foresight!

Do you know what kind of environment these diatoms come from? Are they aquatic or terrestrial? Maybe that has something to do with size.

Very nice, but personally I prefer the ones without the bright orange backlight though (for example, the third from the end). That orange color and high brightness does not go well with the darker greens and blues for me.

Very micely done. I wish I could find "big" diatoms like those. Mine are barely visible even at 50x.

Yes, for all these reasons I do not use postfocus for still subjects. It can be useful for live subjects. But focus bracketing is extremely fast until the buffer fills, so this can be better even for live subjects if the stack is not deep.

Guido, there is an important difference between them. Postfocus gives you less control than bracketing. Sometimes postfocus leaves some foreground out of focus whereas you can precisely pick the zones of focus. At least, that was my experience.

I can see no significant differences in resolution.

That's my conclusion too.

Done. By the way, my specimen is the local South American version of V. cardui; this is Vanessa braziliensis http://www.sangay.eu/frkooxrkwzsowoe.html/fiche-papillon/Nymphalidae/Nymphalinae/Vanessidi/Vanessa/braziliensis+/_ Edit: Just about every inhabited place in the world has this butterfly or it...