Search found 5861 matches

by Charles Krebs
Sun Jul 19, 2020 11:21 pm
Forum: Photography Through the Microscope
Topic: plant leaf stem cross sections
Replies: 12
Views: 856

Re: plant leaf stem cross sections

Hi Charles,.. I've always wondered, does a Vibratome tend to cut with less need for support of the material? I would assume so, especially if the settings were just right. Can one alter the frequency and stroke of the commercial machine? It wouldn't be too difficult to make something with both bein...
by Charles Krebs
Tue Jul 07, 2020 11:48 pm
Forum: Photography Through the Microscope
Topic: Lysichiton americanum (Western Skunk Cabbage) sections
Replies: 3
Views: 295

Lysichiton americanum (Western Skunk Cabbage) sections

These sections are from the central "stalk" of a skunk cabbage plant. Overall the stalk is very "spongy". This is clearly seen in the top two cross section images. You can also see the xylem and phloem tissue interspersed in the first image. The second image is a closer look at this. The third image...
by Charles Krebs
Mon Jun 29, 2020 12:18 am
Forum: Photography Through the Microscope
Topic: plant leaf stem cross sections
Replies: 12
Views: 856

Re: plant leaf stem cross sections

Is it acquired skill or special equipment that results in such perfect sections? (Probably both...) Thanks for the comments. I'm just starting to play around with this subject for myself. I have used a Vibratome at a lab where I work, but at home here all I have is one of those small, inexpensive h...
by Charles Krebs
Mon Jun 22, 2020 10:03 pm
Forum: Photography Through the Microscope
Topic: plant leaf stem cross sections
Replies: 12
Views: 856

Re: plant leaf stem cross sections

sebba28 wrote:
Mon Jun 22, 2020 8:48 pm
Beautiful.What stains did you use?
Thanks.

The stains were toluidine, safranin, and a little combo with Janus green on the 3rd one.
by Charles Krebs
Sun Jun 21, 2020 6:17 pm
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5087

Re: Nikon Diaphot TMD w/ DIC -- Help!

Late to the party here but a few items... I seriously doubt that Nikon just ignored the increase in distance, My TMD came set up for DIC and fluorescence (I don't use it) so I have that ~20mm increase. I would be curious for those who do not have this to look at their nosepieces. My nosepiece (and I...
by Charles Krebs
Sun Jun 21, 2020 4:01 pm
Forum: Photography Through the Microscope
Topic: plant leaf stem cross sections
Replies: 12
Views: 856

plant leaf stem cross sections

Sliced up a few more plants into skinny small pieces :wink: First we have the leaf stalk (petiole) of a lilac bush (Syringa vulgaris). I cut these sections just at the start of the leaf base, where the leaf blade is just beginning to "flare" out. This is what you see as sort of "ears" in the top thr...
by Charles Krebs
Fri Jun 12, 2020 2:29 am
Forum: Photography Through the Microscope
Topic: Broccolini cross section,. Iris stem cross section
Replies: 7
Views: 512

Re: Broccolini cross section,. Iris stem cross section

... All were darkfield illumination... Perfect ! Simple DF only ? No hidden tricks ? Some staining, polarisation etc ? Sorry about that. No intention of hiding anything. These were indeed stained in a very basic manner. (I suppose I thought that would be apparent). The sections were cut with a hand...
by Charles Krebs
Wed Jun 10, 2020 11:44 pm
Forum: Photography Through the Microscope
Topic: Broccolini cross section,. Iris stem cross section
Replies: 7
Views: 512

Broccolini cross section,. Iris stem cross section

Came across some of Robert Berdan's excellent plant section shots and was inspired to dig out my old hand microtome. I raided the veggie compartment for some broccolini (cross between broccoli and Chinese broccoli), and then picked an Iris from my yard. I managed to get some shots and only cut my fi...
by Charles Krebs
Wed Jun 10, 2020 11:24 pm
Forum: Photography Through the Microscope
Topic: cyclops
Replies: 12
Views: 753

Wow! Nicely done.
by Charles Krebs
Wed Jun 10, 2020 11:22 pm
Forum: Photography Through the Microscope
Topic: polychaete trochophore, foraminifer, Strombidium, diatoms
Replies: 11
Views: 715

Thanks for the kind remarks!

For me, looking through a new marine sample is always fascinating and usually presents creatures I've not seen before.
by Charles Krebs
Wed Jun 10, 2020 11:15 pm
Forum: Photography Through the Microscope
Topic: Rotifers, Stylonchia, Gastrotricha & Amoebae > Update
Replies: 5
Views: 530

Walter, you forgot to mention the Paramecium bursaria!

Really nice images from a productive collecting trip.
by Charles Krebs
Wed Jun 10, 2020 11:10 pm
Forum: Photography Through the Microscope
Topic: Peony pollen
Replies: 1
Views: 346

Nice. Reminiscent of Lily pollen if I remember correctly.
by Charles Krebs
Wed Jun 10, 2020 11:09 pm
Forum: Photography Through the Microscope
Topic: Infinite Fluorescence
Replies: 42
Views: 2099

Beautiful images, from an obviously successful (and ambitious) modification.
Congratulations!
by Charles Krebs
Tue Jun 02, 2020 5:45 pm
Forum: Photography Through the Microscope
Topic: polychaete trochophore, foraminifer, Strombidium, diatoms
Replies: 11
Views: 715

polychaete trochophore, foraminifer, Strombidium, diatoms

From recently collected alga (low tide) and a quick plankton net drag. Puget Soound, WA. The first is a polychaete trochophore. This bristle-worm larval stage is planktonic and known as a "trochophore". Very active. Here is a heads on view with electronic flash to stop the action. Olympus 20/0.70 S ...
by Charles Krebs
Tue Jun 02, 2020 4:09 pm
Forum: Photography Through the Microscope
Topic: Pond life
Replies: 8
Views: 511

These (and your other recent post) are great!
I find the 6th one here, daphnia, really intriguing. Very dramatic and like an alien creature from a sci-fi movie!