Garden variety autofluorescence

Images made through a microscope. All subject types.

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pbraub
Posts: 91
Joined: Fri Feb 02, 2018 1:23 pm

Garden variety autofluorescence

Post by pbraub »

Hi,

i am toying around with my new UV excitation autofluorescence setup and took some garden variety images.

Twig of a privet hedge (UPlanFL4x).
Image

Ghostberry (UPlanFL4x).
Image


The last image is a berberis berry with an incidental fungal infection taken with a UPLanFLN10x objective. The fungus was not visible with the naked eye and only revealed itself by its strong blue-ish autofluorescence.
Image

The images were taken with PlanFL 4x and 10x lenses on an Olympus BX61 controlled with Micro-Manager 2 gamma. Illumination came from a UV LED (CoolLED pE300) with a Semrock DAPI EX/BS and a 408 nm LP EM filter. The camera was a Point Grey color camera.

Images were stacked in Zerene (i used the holiday deal, thank you!).

My next plans are to establish size calibration and optimize the stacking protocols.

I hope you like my images and I am looking forward to constructive criticism am discussion.

Kind regards
Peter

carlos.uruguay
Posts: 5358
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
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Post by carlos.uruguay »

Super!

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