Lilly pollen

Images made through a microscope. All subject types.

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JH
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Lilly pollen

Post by JH »

Lilly pollen, Nikon BD Plan Apo 150x NA 0.90. For this picture I used darkfield light. The high NA makes every dot, spot, line... on the sensor visible so I did some retouching both before and after stacking. I darkened the background and sharpened the picture. Canon 5D II, Zerene Stacker, Adobe LR and PS.

Best regards
Jörgen Hellberg


Image
Image
Jörgen Hellberg, my webbsite www.hellberg.photo

rjlittlefield
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Post by rjlittlefield »

Spectacular!

So, this was illuminated with light coming up through the specimen?

--Rik

JH
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Post by JH »

rjlittlefield wrote:Spectacular!

So, this was illuminated with light coming up through the specimen?

--Rik
Thanks!

It is a Bright field/Darkfield (BD) EPI-light lens so light from above.

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Saul
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Post by Saul »

Jörgen , incredible photos, especially stereo.
What setup you are using ?
Thanks,

rjlittlefield
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Post by rjlittlefield »

JH wrote:It is a Bright field/Darkfield (BD) EPI-light lens so light from above.
Ah, epi darkfield. Thanks!

--Rik

Lou Jost
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Post by Lou Jost »

Really beautiful! These kinds of pictures would be great tools for studying pollen samples when the species is unknown, especially ancient pollen.

JH
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Post by JH »

Saul wrote: What setup you are using ?
Hi Saul, I used a Nikon Optiphot microscope. The microscope have a 2.5x projection lens, so a lot of empty magnification and 375x on sensor. The 150x lens only have 0.3 mm working distance.

In this case I wanted to show the colours of the pollen grain. For ”clean” black and white pictures I think that a GIF (green) filter is a good option.

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Jacek
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Post by Jacek »

Nice :D

JH
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Post by JH »

Thanks for the comments!

This is with the 40x BD Plan apo lens, cropped.

Best regards
Jörgen Hellberg


Image

Image
Jörgen Hellberg, my webbsite www.hellberg.photo

Olympusman
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Lily Pollen

Post by Olympusman »

That is crazy beautiful. Really loved the 3D.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

WalterD
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Post by WalterD »

Hi Jorgen,

Very interesting and aesthetic. The 40x red ones are my favourite!
Thanks for sharing.

JH
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Post by JH »

Thanks Mike and WalterD!

Here is a link to a Video https://youtu.be/s9Eh4rDLE_8

I used the new Zerne Stacker function Stere/Rocking "Use conical path"

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Marek Mis
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Post by Marek Mis »

Nice images Jorgen ! The red ones remind me slightly odd strawberries :)

Marek

JH
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Post by JH »

Thanks Marek

Here is a panorama made from four stacked pictures. I used the BD plan apo 150x lens.

Best regards
Jörgen Hellberg

Image
Jörgen Hellberg, my webbsite www.hellberg.photo

Beatsy
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Post by Beatsy »

Spectacular! Practically a separate genre - photomiacrography, or miacro for short :)

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