Living creatures under reflected light microscope?

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Jesse
Posts: 69
Joined: Sun Dec 11, 2016 6:40 pm

Living creatures under reflected light microscope?

Post by Jesse »

Looking at microscopes, I see that Epi microscopes come with quite high magnifications.

Curiously, I don't think I've ever seen living creatures under a reflected light microscope. Why is that? What does pod life look like under a reflected light microscope?

Alan Wood
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Joined: Wed Dec 29, 2010 3:09 pm
Location: Near London, U.K.
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Post by Alan Wood »

Jesse

Depth of field is very small, you get a much better view with a stereomicroscope.

Dark-ground reflected light works fine for biological specimens, apart from the minimal depth of field.

Bright-field reflected light with biological specimens can give very poor results with lots of flare. Adjusting a polariser and analyser can help a bit.

Alan Wood

Jesse
Posts: 69
Joined: Sun Dec 11, 2016 6:40 pm

Post by Jesse »

Alan Wood wrote:Jesse

Depth of field is very small, you get a much better view with a stereomicroscope.

Dark-ground reflected light works fine for biological specimens, apart from the minimal depth of field.

Bright-field reflected light with biological specimens can give very poor results with lots of flare. Adjusting a polariser and analyser can help a bit.

Alan Wood
Can you provide some example photos? I'm just horribly curious.

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