Barnacles, auto-fluorescence. (Stereo added)

Images made through a microscope. All subject types.

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Charles Krebs
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Barnacles, auto-fluorescence. (Stereo added)

Post by Charles Krebs »

10X, 20X, and 5X Olympus M Plan Fluorites. Two lights with 365nm Nichia LEDs. 435nm barrier filter.

Image

Image

Image
Last edited by Charles Krebs on Tue May 09, 2017 9:04 pm, edited 1 time in total.

Jacek
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Post by Jacek »

SUPER !

Marek Mis
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Post by Marek Mis »

Wonderful images Charlie !

What the working distances have these objectives ?

Marek

WalterD
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Post by WalterD »

A complicated subject with fantastic details, Charles, very impressive!

JH
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Post by JH »

Artistic and interesting.
Best regards Jörgen
Jörgen Hellberg, my webbsite www.hellberg.photo

Charles Krebs
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Post by Charles Krebs »

Stereo from 68 image stack. Olympus 10/0.30 MPLFLN. It is a little surprising to me how "cleanly" a complex, "hairy" image like this stacks with auto-fluorescence. (In this case I have an air bubble and a bit of debris that I cleaned up easily on the 2D image that I will not attempt to edit on the stereo. :) )

Image

Charles Krebs
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Post by Charles Krebs »

Marek Mis wrote:What the working distances have these objectives?
5/0.15 MPLFLN : 20mm
10/0.30 MPLFLN : 11mm
20/0.40 LMPLFLN : 12mm
50/0.50 LMPLFLN : 10.6mm

Saul
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Post by Saul »

Impressive stereo. Air bubble is a plus, brings more 3D :) ...
Saul
μ-stuff

elf
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Post by elf »

What are the red objects? Eggs of some sort?

carlos.uruguay
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Post by carlos.uruguay »

Nice!

Pau
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Post by Pau »

Excellent again!
If you desaturate them they look like SEM.
The stereo is awesome.
elf wrote:What are the red objects? Eggs of some sort?
Algae, likely diatoms (chlorophyll emits strong red fluorescence)
Pau

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