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Amoeba test

 
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Fri Jul 13, 2007 11:32 pm    Post subject: Amoeba test Reply with quote

These are always a constant source of amazement to me! Microscopic "pottery" made by an amoeba!

Olympus 40/0.95 S Plan Apo, Canon 350D, bright-field illumination (slightly oblique), 24 stacked images using Helicon Focus
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beetleman



Joined: 04 Aug 2006
Posts: 3578
Location: Southern New Hampshire USA

PostPosted: Sat Jul 14, 2007 11:21 am    Post subject: Reply with quote

And you always give us the best 3D views of them with your fantastic stacks Charles.
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Wolfgang Bettighofer



Joined: 11 Mar 2007
Posts: 58
Location: Kiel, Germany

PostPosted: Sat Jul 14, 2007 3:18 pm    Post subject: Re: Amoeba test Reply with quote

Charles Krebs wrote:
These are always a constant source of amazement to me! Microscopic "pottery" made by an amoeba!



Hi Charles,

very nice! You did a great job in parameterizing the stacking software. But what amoeba? The surface looks like Difflugia and the shape like Lesquereusia.

So long, Wolfgang
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Sun Jul 15, 2007 9:35 am    Post subject: Reply with quote

Quote:
The surface looks like Difflugia and the shape like Lesquereusia.

Yes, I thought that very same thing! I have seen such amazing variations of these tests. It really is a shame they are so difficult to photograph well. Stacking is a big help, but my biggest problem has been the "depth" required by the subject and my inability to get a nice sharp image (of the deeper sections) due to spherical aberration. And this is even with a 40X that has a correction collar. I'm beginning to think that I should try to collect some specimens and try it "dry" with a 40X objective designed for use without a coverslip.
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Wolfgang Bettighofer



Joined: 11 Mar 2007
Posts: 58
Location: Kiel, Germany

PostPosted: Sun Jul 15, 2007 2:16 pm    Post subject: Reply with quote

Charles Krebs wrote:
Quote:
The surface looks like Difflugia and the shape like Lesquereusia.

... I'm beginning to think that I should try to collect some specimens and try it "dry" with a 40X objective designed for use without a coverslip.


Looking at your picture I have no idea what could be done better... Smile
Some words to objectives designed for use without a coverslip. Are there any for brightfield use? In former times I used a 40/0.95 objective with correction collar. For me this was not satisfying. Since the time I'm using a 40/1.00 oil I'm happy.
Have you ever tested a 40x oil?

Cheers, Wolfgang
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Planapo



Joined: 07 Nov 2006
Posts: 1533
Location: Germany, in the United States of Europe

PostPosted: Wed Jul 18, 2007 1:35 pm    Post subject: Reply with quote

Gorgeous image, with respect to biological documentation as well as artistic value, Charles. It displays on my monitor so crisply and three-dimensionally as if I just could grab it.

Truly, never have I seen a better picture of Testacean "pottery" than this before. Thanks for showing.

Cheers,
Betty
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Thu Jul 19, 2007 10:21 am    Post subject: Reply with quote

Wolfgang...
Quote:
Have you ever tested a 40x oil?

I have a 40X Zeiss Plan Apo oil. Unfortunately it has some of the dreaded "delamination" so common in those objectives. It still provides a very good image, and does make things simpler as far as not needing to fiddle around with an adjustment collar. But I am not convinced that it really is better when a subject is rather "deep" in the water under the coverglass.
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Wolfgang Bettighofer



Joined: 11 Mar 2007
Posts: 58
Location: Kiel, Germany

PostPosted: Fri Jul 20, 2007 3:37 pm    Post subject: Reply with quote

Charles Krebs wrote:
Wolfgang...
Quote:
Have you ever tested a 40x oil?

I have a 40X Zeiss Plan Apo oil. Unfortunately it has some of the dreaded "delamination" so common in those objectives. It still provides a very good image, and does make things simpler as far as not needing to fiddle around with an adjustment collar. But I am not convinced that it really is better when a subject is rather "deep" in the water under the coverglass.


OK, good to know that one can handle the 40/0,95. I gave it up, years ago, but you show that one can handle.
But I think that the appr. homogenous immersion could make it possible to image subjects in deep water a little better than "dry" objectives (I don't know the correct expression). Unfortunately I'm not able to test...

Cheers, Wolfgang
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