Just noticed what you said now. Those are some interesting points... I do not believe that specular highlights are an issue with confocal because the light source is large and coming from many different angles. With light sheet maybe, depending on how the sheet is set up. With 'real' light sheet, a laser is used to create a microscopically thin slice of light, but for our purposes, the sheet could be as big as half of the depth of the subject and still at least partially function by preventing light from hitting parts of the subject that we aren't trying to photograph in those frames.perdu34 wrote:
I may be getting the wrong end of the stick here but I would think this is a really bad idea for macrophotography.
I'm not totally sure what you mean by separating the input light from the reflected light, because it is definitely possible to use a beamsplitter to illuminate the subject in full color, through the objective. It is true that dichroics don't really apply, because there is no fluorescence involved.