Paramecium caudatum

sushidelic · Post by **sushidelic** » Tue Apr 10, 2018 7:42 am

Olympus UPLANAPO 40x, U-DICT, 1/2000sec, no flash...

Best regards,
Michael

vasselle · Post by **vasselle** » Tue Apr 10, 2018 3:36 pm

Magnifique

micro_pix · Post by **micro_pix** » Tue Apr 10, 2018 4:52 pm

Wonderful photos!

How do you get 1/2000 sec with no flash in DIC with a 40X objective? It is very impressive.

razashaikh · Post by **razashaikh** » Tue Apr 10, 2018 9:44 pm

Very nice observation.

Jacek · Post by **Jacek** » Wed Apr 11, 2018 4:33 am

Very nice

Cactusdave · Post by **Cactusdave** » Thu Apr 12, 2018 12:20 am

Beautiful, I'd like to repeat micro_pix's question

How do you get 1/2000 sec with no flash in DIC with a 40X objective? It is very impressive.

Only I was thinking "How the expletive do you get ..... etc".

sushidelic · Post by **sushidelic** » Thu Apr 12, 2018 1:32 am

Hi and thanks for your answers!

As I do not have a flash adaption yet (biiiig thing on the wish list), I am forced to use the continuous light of the scope.
Here it was a BX50 with a U-LH100 lamp house, so 100W halogen.

For pond peeping pictures, I remove all filters (ND, LBD...) from the light path and set the illumination power to max. Color temperature is then compensated by white balancing the camera. The only "light loss" is the trinocular, as I still direct 20% of the light to the eyepieces and 80% to the cam.

I use a Nikon D800 for taking the pictures, on a U-SWTR-2 tube and project directly to the sensor, no projective used. Certainly this results in a vignetted image on a FF sensor, but more light is directed towards one pixel due to the smaller image spot.

DIC is set in a way that still a lot of light passes the filters (so not too close to "DF / crossed nicols", but DIC relief is still satisfying.

With all the above I am able to use continuous light, 40x and DIC with ISO ~1200/1600 and exposure times of 1/1000 - 1/2000 seconds.
This is needed in two regards - freezing the specimen, cilia etc. AND to prevent the earthquake like mirror shock of the D800 when used without exposure delay.

Hope this helps and best regards,
Michael

micro_pix · Post by **micro_pix** » Thu Apr 12, 2018 3:23 am

Thanks for the explanation. I use a BH2 BHS so I do have the 100w halogen but I do use the projection eyepiece. I must admit I haven’t used the high asa settings as I thought there would be too much noise but I’ll give that a try.

David

Cactusdave · Post by **Cactusdave** » Fri Apr 13, 2018 7:09 am

My thanks too Michael. You are lucky to have the combination of a really powerful light source and a camera with an excellent high ISO performance. Great work, and proves it is possible to get this kind of image without flash.

Robert Berdan · Post by **Robert Berdan** » Tue May 01, 2018 10:40 am

Beautiful pictures. To reduce vibration on your D800 you might try using Live View and Digicam control link to a PC - the software is free. I also take pictures at high ISO speeds and reduce the grain considerably using Adobe Camera Raw by reducing luminance noise and I get good results up to ISO 6400 on my D800 and D500. I also use a 100W Halogen light source.

I am experimenting with setting up a flash on my Axioscope using a beam splitter. The problem is I only get about 50% of the Flash or less to the speciment and there is no room to place the flash below the condensor so a beam splitter seems like the only way I can do this. If Nikon had an Automatic flash that would sit flat on top of the light source that might work better, but then I couldn't see what I am photographing. I am working on a solution hope to have it figured out in a few weeks.

Pau · Post by **Pau** » Tue May 01, 2018 11:46 am

Robert Berdan wrote: I am experimenting with setting up a flash on my Axioscope using a beam splitter...

Did you see?
http://www.photomacrography.net/forum/v ... p?p=161563

hkv · Post by **hkv** » Sat May 05, 2018 9:09 am

Beautiful shots!

carlos.uruguay · Post by **carlos.uruguay** » Wed May 09, 2018 6:34 am

Nice!!!

Smokedaddy · Post by **Smokedaddy** » Wed May 09, 2018 2:15 pm

Very nice images Michael.

Paramecium caudatum

Paramecium caudatum

Nice DIC paramecium Photos

Re: Nice DIC paramecium Photos