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Microtomy school

 
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Olympusman



Joined: 15 Jan 2012
Posts: 3568

PostPosted: Fri Apr 13, 2018 8:33 am    Post subject: Microtomy school Reply with quote

I had the opportunity the other day to visit the histology department at a medical center to learn what I could about microtomy. Absolutely fascinating. Mainly I learned a great deal about the importance of temperature at various stages of tissue preparation and embedding. Got to watch a cryomicrotome in action. Also learned about dehydrating tissue samples. Instead of progressively stronger steps in alcohol the technique they use is
10% buffered Formalin
70% Formalin 30% alcohol
Pure alcohol
Pure alcohol
Pure alcohol
Xylene

I also learned about prepared ionized slides which are used with silver stained tissues (brain, bone). Silver stained tissues don't adhere to normal slide and the tissue washes off during staining.
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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ChrisR
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Joined: 14 Mar 2009
Posts: 7607
Location: Near London, UK

PostPosted: Fri Apr 13, 2018 2:15 pm    Post subject: Reply with quote

I learned enough about it to be fascinated and, inevitably, accumulate some hardware. I doubt I have enough life left to actually get round to practicing, though!
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Chris R
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Olympusman



Joined: 15 Jan 2012
Posts: 3568

PostPosted: Sat Apr 14, 2018 1:09 pm    Post subject: Microtomy Reply with quote

Got out my binder of notes I accumulated over the years to review technique.
Purchased 10% buffered formalin and Potassium hydroxide. Also purchased a digital kitchen thermometer to establish heating of Paraplast and water bath atop a gravy warmer and a coffee cup warmer.
This is getting complicated for a hobby microscopist.
More hardware with a fixed amount of counter space in the lab.

Mike
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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ChrisR
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Location: Near London, UK

PostPosted: Sun Apr 15, 2018 2:45 pm    Post subject: Reply with quote

It's also expensive. Minimum qty seems to be about $100.

I saw a Leitz microtome and bought it, expecting imagination to be fired. Still waiting.
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Chris R
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Olympusman



Joined: 15 Jan 2012
Posts: 3568

PostPosted: Mon Apr 16, 2018 2:01 pm    Post subject: Buffered formalin Reply with quote

The histology lab I visited had a 2.5 gallon cubitainer of formalin. I purchased two pair of jars that appear to hold about 40 or 50 ml on Amazon from TissuePro Technology www.tissueprotech.com

As for the KOH, I now have a lifetime supply. I found it is very effective at dissolving residue accumulated in beakers when I decapsulate microchips.

Mike
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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