An improved fluorescence set up

Images made through a microscope. All subject types.

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Nezza
Posts: 27
Joined: Tue Oct 14, 2014 8:07 am
Location: UK

An improved fluorescence set up

Post by Nezza »

I have been playing with my fluorescence set up recently. To date I have built a simple 1W blue LED lightsource and I have made my 445nm laser set up safer. I have a new laser module on a heatsink with a low power lens so the beam diverges nicely when I plug it into the lamp holder on my Olympus microscope. I use dark ground illumination and a 450nm or 500nm blocking filter. The advantage of using a laser is that it is monochromatic so even the 450nm blocking filter gets rid of almost all of the stray blue light. Anyway here are a couple of the preliminary images.

1. is an old prep of daffodil pollen (unstained).

2 & 3 are bacteria stained with acridine orange.

The images were photographed using an EOS 6D at 1600 ISO and exposures of 4 to 8 seconds.

Image

Image

Image

Ken Ramos
Posts: 7208
Joined: Thu Jul 27, 2006 2:12 pm
Location: lat=35.4005&lon=-81.9841

Post by Ken Ramos »

Pretty highbrow to me but interesting to see all the same. Wonderful! 8)

Pitufo
Posts: 260
Joined: Sun Jun 21, 2015 4:32 am
Location: United Kingdom

Post by Pitufo »

These are very interesting, thanks for sharing.

I was thinking of having a go at something similar myself. Could you give us a bit more information about your blue light and cost/where to buy in UK?

carlos.uruguay
Posts: 5358
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Nice

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