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Utricularia - inside the bladder of doom
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Sun Nov 30, 2014 3:02 pm    Post subject: Utricularia - inside the bladder of doom Reply with quote

Greetings! I'd like to share with you some confocal images of the trap (or bladder) of an aquatic carnivorous plant, Humped bladderwort (Utricularia gibba). Enjoy!

Magnification: 100x
Microscope: Zeiss confocal laser scanning microscope LSM 710.
Objective: 10x, NA 0.45
Laser lines: 405, 488, 562 nm.
Colors: blue and green - cellulose stained with Calcofluor white; red - autofluorescence of chloroplasts.







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sebba28



Joined: 29 Dec 2011
Posts: 274
Location: Poland,Ireland

PostPosted: Sun Nov 30, 2014 3:11 pm    Post subject: Reply with quote

Beautiful images.
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Sumguy01



Joined: 28 Jan 2013
Posts: 1356
Location: Ketchikan Alaska USA

PostPosted: Sun Nov 30, 2014 6:34 pm    Post subject: Reply with quote

Very Happy Very interesting pics.
Thanks for sharing.
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GemBro



Joined: 20 Aug 2014
Posts: 261
Location: Surrey [UK]

PostPosted: Sun Nov 30, 2014 8:27 pm    Post subject: Reply with quote

Blimey Shocked ... fantastic shots ...
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Cactusdave



Joined: 09 Jun 2009
Posts: 1631
Location: Bromley, Kent, UK

PostPosted: Mon Dec 01, 2014 1:40 am    Post subject: Reply with quote

Laser scanning confocal is just great, the red Ferrari supermodel of light microscopy Laughing Great images, no more to say. Didn't I see that one of the second set won an award recently? Wink
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ChrisR
Site Admin


Joined: 14 Mar 2009
Posts: 8369
Location: Near London, UK

PostPosted: Mon Dec 01, 2014 3:07 am    Post subject: Reply with quote

Astonishing. Thanks for showing these. A load of questions arise Smile
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escocat



Joined: 19 Nov 2014
Posts: 273
Location: Messina - Sicily - Italy

PostPosted: Mon Dec 01, 2014 3:12 am    Post subject: Reply with quote

The second is a real show Shocked
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5249
Location: Uruguay - Montevideo - America del Sur

PostPosted: Mon Dec 01, 2014 3:39 am    Post subject: Reply with quote

Impressive!!!!
How are the organisms prepared to observe them?
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Jacek



Joined: 02 Oct 2011
Posts: 5342
Location: Poland

PostPosted: Mon Dec 01, 2014 4:23 am    Post subject: Reply with quote

fabulous pictures, collect jaw from the floor Shocked
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arturoag75



Joined: 05 Feb 2010
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Location: italy

PostPosted: Mon Dec 01, 2014 4:26 am    Post subject: Reply with quote

Absolutely fantastic Shocked Shocked
Arturo
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RogelioMoreno



Joined: 20 Nov 2009
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Location: Panama

PostPosted: Mon Dec 01, 2014 5:48 am    Post subject: Reply with quote

I love all! Shocked

Rogelio
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Mon Dec 01, 2014 8:31 am    Post subject: Reply with quote

carlos.uruguay wrote:
Impressive!!!!
How are the organisms prepared to observe them?


Thanks, Carlos. The traps were fixed in 2% formaldehyde/2% glutaraldehyde in pond water, stained with Calcofluor White, embedded in 6% agarose, sectioned with Vibratome @ 0.5 mm in 2 planes (frontal, sagital), mounted in slightly alkaline water.
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Mon Dec 01, 2014 8:33 am    Post subject: Reply with quote

Jacek wrote:
fabulous pictures, collect jaw from the floor Shocked


Dziekuje, Jacek! Gdzie w Polsce przebywasz? Ja jestem z Krakowa Smile
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Olympusman



Joined: 15 Jan 2012
Posts: 4193

PostPosted: Mon Dec 01, 2014 8:44 am    Post subject: Bladderwort Reply with quote

Zowie!
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Mon Dec 01, 2014 8:46 am    Post subject: Reply with quote

Cactusdave wrote:
Laser scanning confocal is just great, the red Ferrari supermodel of light microscopy Laughing Great images, no more to say. Didn't I see that one of the second set won an award recently? Wink


Yes, LSCM is a powerful tool - but when it comes to output images I'm finding myself drawn to the aesthetics of the Nomarski DIC microscopy lately Smile

It was a similar one - see below.

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